Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems.
Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems.
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DOI：
10.1002/cbic.201500340
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Kubick S
中科院分区：
文献类型：
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作者：
Zemella A;Thoring L;Hoffmeister C;Kubick S
从一开始作为一个小规模的体外系统来研究基本的翻译过程，无细胞蛋白质合成迅速成为一个高产量生产蛋白质的有效平台。与经典的体内蛋白质表达相反，无细胞系统不需要耗时的克隆步骤，并且开放的性质提供了易于操作的反应条件以及高通量潜力。特别是对于难以表达的蛋白质的合成，如毒性和跨膜蛋白，无细胞系统是巨大的兴趣。特别是将非规范氨基酸掺入靶蛋白的遗传密码的修饰在生物技术和药物研究中提供了巨大的潜力，并且是许多无细胞项目的焦点。已经建立了许多用于多种应用的复杂的无细胞系统。本文综述了无细胞蛋白质合成的最新进展，并详细介绍了该领域的应用前景。
From its start as a small-scale in vitro system to study fundamental translation processes, cell-free protein synthesis quickly rose to become a potent platform for the high-yield production of proteins. In contrast to classical in vivo protein expression, cell-free systems do not need time-consuming cloning steps, and the open nature provides easy manipulation of reaction conditions as well as high-throughput potential. Especially for the synthesis of difficult to express proteins, such as toxic and transmembrane proteins, cell-free systems are of enormous interest. The modification of the genetic code to incorporate non-canonical amino acids into the target protein in particular provides enormous potential in biotechnology and pharmaceutical research and is in the focus of many cell-free projects. Many sophisticated cell-free systems for manifold applications have been established. This review describes the recent advances in cell-free protein synthesis and details the expanding applications in this field.