An alloantigen selective for B cells: Ly-17.1.
An alloantigen selective for B cells: Ly-17.1.
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对 B 细胞具有选择性的同种异体抗原：Ly-17.1。
DOI：
10.1007/bf01567798
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发表时间：
1980
期刊：
影响因子：
3.2
通讯作者：
Boyse,EA
中科院分区：
文献类型：
--
作者：
Shen,FW;Boyse,EA
同种异体抗原Ly-17.1由抗血清（C_3Hf/Bi x B_6-Tla a）抗C_3H/An脾（~-Ly-17.1）鉴定。细胞毒试验不能证明这种细胞表面抗原，本文所给的数据是用15份c~-Ly-17.1血清样品（在第6次至第20次接种后采集）获得的，所有这些样品经PA-SRBC试验均为阳性，而细胞毒试验均为阴性。PA-SRBC试验中，SRBC与蛋白A结合，形成抗体包被细胞的抗体，按照Koo和Goldberg（1978）最初的描述进行。C3 H/An小鼠中Ly-17+（玫瑰花状）细胞的最大百分比计数为：脾脏，39+ _5;淋巴结，22+ _3;骨髓，21+ _3;胸腺，2+ 2（e-Ly-17.1血清稀释度高达1：40; 1 x 107个细胞/ml; 4只小鼠的结果平均值）。Ly-17.1+菌株之间的计数无显著差异（如下所列）。Ly-17.1的PA-SRBC测定法的优点是对照计数（Ly-17.1-小鼠的脾细胞，如下所列）总是几乎为零，因此在很大程度上避免了校正的需要。表1中的数据支持这一结论，因为：（a）可能所有的Ly-17 §细胞都粘附在尼龙上，（B）Lyt-1 §细胞（占脾Thy-1 §细胞的90- 95%）的消除使脾Ly-17 §细胞群富集到与尼龙洗脱相同的程度。
Alloantigen Ly-17.1 is identified by the antiserum (C3Hf/Bi x B6-Tla a) anti C3H/An spleen (~-Ly-17.1). This cell-surface antigen has not been demonstrable by cytotoxicity assay; the data given here were obtained with a pool of 15 c~-Ly-17.1 serum samples (taken after the 6th to 20th inoculations), all of which were positive by PA-SRBC assay but negative by cytotoxicity assay. The PA-SRBC assay, in which SRBC conjugated to protein A form rosettes with antibody-coated cells, was performed as originally described by Koo and Goldberg (1978). Maximum percentage counts of Ly-17+(rosetted) cells in C3H/An mice were: spleen, 39+ _5; lymph nodes, 22+ _3; bone marrow, 21+ _3; thymus, 2+ 2 (e-Ly-17.1 serum dilutions up to 1: 40; 1 x 107 cells per ml; mean of results for four mice). There were no significant differences in counts among Ly-17.1+ strains (listed below). A virtue of the PA-SRBC assay for Ly-17.1 is that control counts (spleen cells of Ly-17.1-mice, listed below) are always virtually nil, thus largely obviating the need for corrections.The counts given above may suggest that B cells are Ly-17+ and T cells Lyl7-. The data in Table 1 support this conclusion because:(a) probably all Ly-17 § cells adhere to nylon, and (b) elimination of Lyt-1 § cells, comprising 90-95 percent of splenic Thy-1 § cells, enriches the splenic Ly-17 § population to about the same degree as elution from nylon.