Endothelium-dependent pressure-induced contraction of isolated canine carotid arteries.
Endothelium-dependent pressure-induced contraction of isolated canine carotid arteries.
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内皮依赖性压力引起的离体犬颈动脉收缩。
DOI：
10.1152/ajpheart.1988.255.4.h783
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Rubanyi,GM
中科院分区：
文献类型：
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作者：
Rubanyi,GM
实验在生物测定系统中进行，其中没有内皮的犬冠状动脉环（生物测定组织）被来自灌注有内皮的犬颈动脉段（供体段）的流出物灌注。跨壁压力的快速增加（从接近 0 到 32-38 mmHg）触发供体节段的主动收缩，同时触发生物测定组织的主动收缩。这些收缩可以通过从供体节段去除内皮来防止，但不能通过用吲哚美辛处理该节段来防止。暴露于高压会抑制基础、乙酰胆碱和血流诱导的内皮衍生舒张因子的释放。亚甲蓝可防止压力引起的生物测定环收缩。这些数据表明，压力诱导的离体颈动脉收缩是内皮依赖性的，并且是通过抑制内皮衍生的舒张因子的合成和/或释放来介导的。
Experiments were conducted in a bioassay system, where a canine coronary artery ring without endothelium (bioassay tissue) was superfused by the effluent from a perfused canine carotid artery segment with endothelium (donor segment). A rapid increase in transmural pressure (from near 0 to 32-38 mmHg) triggered active contraction of the donor segment and simultaneously of the bioassay tissue. These contractions were prevented by removal of the endothelium from the donor segment but not by treatment of the segment with indomethacin. Exposure to elevated pressure depressed basal, acetylcholine-, and flow-induced release of endothelium-derived relaxing factor(s). Methylene blue prevented the pressure-induced contraction of the bioassay ring. These data show that pressure-induced contraction of isolated carotid arteries is endothelium dependent and is mediated by the depression of the synthesis and/or release of endothelium-derived relaxing factor(s).