1,10-Phenanthroline-cuprous ion complex, a potent inhibitor of DNA and RNA polymerases.
1,10-Phenanthroline-cuprous ion complex, a potent inhibitor of DNA and RNA polymerases.
复制标题
1,10-菲咯啉-亚铜离子复合物，DNA 和 RNA 聚合酶的有效抑制剂。
DOI：
10.1016/0006-291x(78)91348-7
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发表时间：
1978
影响因子：
3.1
通讯作者：
D. Sigman
中科院分区：
文献类型：
--
作者：
Vito D'Aurora;A. Stern;D. Sigman
Abstract The inhibition by 1, 10-phenanthroline of E. coli DNA polymerase I has recently been attributed to the formation in the assay mixtures of a unique and effective inhibitor, the 2: 1 1, 10-phenanthroline-cuprous ion complex (1). We have now found that this coordination complex is also an effective inhibitor of E. coli DNA dependent RNA polymerase, Micrococcus luteus DNA dependent DNA polymerase, and T-4 DNA dependent DNA polymerase. This conclusion is based either on the requirement of a thiol for 1, 10-phenanthroline inhibition or on the reversal of 1, 10-phenanthroline inhibition by the non-inhibitory cuprous ion specific chelating agent 2, 9-dimethyl-1, 10-phenanthroline. 2, 2′, 2 ″-Terpyridine is also very effective at relieving 1, 10-phenanthroline inhibition. The reversal of 1, 10-phenanthroline inhibition should be attempted before it is claimed that 1, 10-phenanthroline inhibits any polymerases by coordinating a zinc ion at the active site.
