Zebrafish embryo bioassays for a comprehensive evaluation of microalgae efficiency in the removal of diclofenac from water
Zebrafish embryo bioassays for a comprehensive evaluation of microalgae efficiency in the removal of diclofenac from water
复制标题
DOI：
10.1016/j.scitotenv.2018.05.353
复制
发表时间：
2018-11-01
影响因子：
9.8
通讯作者：
Otero, Marta
中科院分区：
文献类型：
--
作者：
Escapa, Carla;Torres, Tiago;Otero, Marta
本工作涉及的初步研究，旨在评估三种不同的微藻菌株，即小球藻sorokiniana，小球藻和栅藻在双氯芬酸污染水体的生物修复的效率。为此，微藻培养鼓泡柱光生物反应器（PBRs）下分批操作，直到指数生长期结束。3株菌对双氯芬酸的去除均表现为沿着微藻生长而降低，表明生物降解是其主要的去除机制。3株菌株中，S. Alcohus最能降低双氯芬酸浓度（从25，000 μ g l（-1）的初始浓度去除99%）。然而，这样大的去除量不能保证有效的处理，因为在双氯芬酸的生物降解过程中可能产生超过母体化合物的浓度和/或毒性的转化产物（TP）。因此，为了全面评价微藻处理效率，测试了PBRs的最终流出物对斑马鱼胚胎发育的影响。再次，S。处理是最有效的减少毒性，与相应的流出物没有影响胚胎的死亡率或异常的发生率（在受精后80小时）。在任何情况下，对于三种菌株，流出物的毒性效应等于或低于相同浓度双氯芬酸溶液的毒性效应。因此，可以说，在分批培养结束时，通过所考虑的菌株去除双氯芬酸不涉及对斑马鱼胚胎产生毒性TP。(c)2018 Elsevier B. V.版权所有。
This work deals with a preliminary study aimed at evaluating the efficiency of three different microalgae strains, namely Chlorella sorokiniana, Chlorella vulgaris and Scenedesmus obliquus in the bioremediation of diclofenac contaminated water. For this purpose, microalgae were cultured in bubbling column photobioreactors (PBRs) under batch operation until the end of the exponential growth phase. For the three strains, the concentration of diclofenac in the PBRs aquatic medium decreased along microalgae growing, which pointed to biodegradation as the main removal mechanism. Among the three strains, S. obliquus was the most capable to reduce diclofenac concentration (99% removal from an initial concentration of 25,000 mu g l(-1)). However, such a large removal does not guarantee an efficient treatment since transformation products (TPs) exceeding the concentration and/or toxicity of the parent compound may be generated during biodegradation of diclofenac. Thus, for a comprehensive evaluation of the microalgae treatments efficiency, the final effluents from the PBRs were tested for their effects on the embryonic development of zebrafish. Again, the S. obliquus treatment was the most efficient in the reduction of toxicity, with the corresponding effluents having no effects on the embryo's mortality or abnormalities incidence (at 80 h post fertilization). In any case, for the three strains, the toxicity effects of effluents were equal or lower than those determined for diclofenac solutions with the same concentration. Therefore, it may be stated that, at the end of the batch culture, the removal of diclofenac by the considered strains did not involve the generation of toxic TPs to zebrafish embryos. (c) 2018 Elsevier B.V. All rights reserved.