THE MULTIDRUG RESISTANCE (MDR1) GENE-PRODUCT FUNCTIONS AS AN ATP CHANNEL
THE MULTIDRUG RESISTANCE (MDR1) GENE-PRODUCT FUNCTIONS AS AN ATP CHANNEL
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DOI：
10.1073/pnas.90.1.312
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发表时间：
1993-01-01
影响因子：
11.1
通讯作者：
CANTIELLO, HF
中科院分区：
文献类型：
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作者：
ABRAHAM, EH;PRAT, AG;CANTIELLO, HF
多药耐药（mdr 1）基因产物，P-糖蛋白，是负责ATP依赖性挤出的各种化合物，包括化疗药物，从细胞。这里提供的数据表明，P-糖蛋白水平增加的细胞释放ATP到培养基中，与其质膜中蛋白质的浓度成比例。此外，全细胞和单通道电流与膜片钳电极的测量表明，P-糖蛋白作为一个ATP传导通道的质膜。这些发现表明P-糖蛋白的一个不寻常的作用。
The multidrug resistance (mdr1) gene product, P-glycoprotein, is responsible for the ATP-dependent extrusion of a variety of compounds, including chemotherapeutic drugs, from cells. The data presented here show that cells with increased levels of the P-glycoprotein release ATP to the medium in proportion to the concentration of the protein in their plasma membrane. Furthermore, measurements of whole-cell and single-channel currents with patch-clamp electrodes indicate that the P-glycoprotein serves as an ATP-conducting channel in the plasma membrane. These findings suggest an unusual role for the P-glycoprotein.