FrzE of Myxococcus xanthus is homologous to both CheA and CheY of Salmonella typhimurium.
FrzE of Myxococcus xanthus is homologous to both CheA and CheY of Salmonella typhimurium.
复制标题
DOI：
10.1073/pnas.87.15.5898
复制
发表时间：
1990-08
影响因子：
11.1
通讯作者：
W. McCleary;D. Zusman
中科院分区：
文献类型：
--
作者：
W. McCleary;D. Zusman
黄色粘球菌表现出多细胞发育。“卷曲”（frz）突变体不能完成发育途径。这些突变体不是形成子实体，而是形成缠结的细胞细丝。我们以前已经表明，四个frz基因产物是同源的肠道趋化蛋白，并提出了frz基因构成一个信号转导途径，控制细胞逆转其滑行方向的频率。我们在这里显示，frzE编码的蛋白质与计算的分子量为83 kDa。FrzE与鼠伤寒沙门氏菌的CheA和CheY同源，它们是“双组分反应调节剂”家族的成员。“据认为，调节剂组分自磷酸化并将磷酸基团转移到其同源效应物组分。FrzE含有一个不寻常的（丙氨酸加脯氨酸）丰富的区域，可能构成一个灵活的铰链，促进磷酸盐功能域之间的转移。我们认为，FrzE是一个第二信使之间的信号蛋白FrzCD和滑翔机的中继信息。
Myxococcus xanthus exhibits multicellular development. The "frizzy" (frz) mutants are unable to complete the developmental pathway. Instead of forming fruiting bodies, these mutants form tangled filaments of cells. We have previously shown that four of the frz gene products are homologous to enteric chemotaxis proteins and have proposed that the frz genes constitute a signal-transduction pathway that controls the frequency at which cells reverse their gliding direction. We show here that frzE encodes a protein with a calculated molecular mass of 83 kDa. FrzE is homologous to both CheA and CheY of Salmonella typhimurium, which are members of a family of "two-component response regulators." It is thought that the modulator components autophosphorylate and transfer a phosphate group to their cognate effector components. FrzE contains an unusual (alanine plus proline)-rich region that might constitute a flexible hinge facilitating phosphate transfer between functional domains. We suggest that FrzE is a second messenger that relays information between the signaling protein FrzCD and the gliding motor.