Sensitive assay for sodium pump inhibition.
Sensitive assay for sodium pump inhibition.
复制标题
钠泵抑制的灵敏测定。
DOI：
10.1093/clinchem/40.8.1595
复制
发表时间：
1994
影响因子：
9.3
通讯作者：
S. Graves
中科院分区：
文献类型：
--
作者：
Q. Tao;P. Soszyński;N. Hollenberg;S. Graves
在乙醇的发射测定中，高LDH和乳酸盐浓度的正干扰是由LDH催化乳酸盐转化为丙酮酸盐引起的，在此期间NAD也转化为NADH。测量340 nm处吸光度的变化以反映NAD向NADH的转化也是乙醇发射测定的基础，但通过乙醇脱氢酶催化乙醇转化为乙醛。通过超滤除去样品中的LDH完全消除了干扰。
In the Emit assay of alcohol, the positive interference from high LDH and lactate concentrations is caused by the catalytic conversion of lactate to pyruvate by LDH, during which also NAD is converted to NADH. Measurement of the change in absorbance at 340 nm to reflect the conver-sion of NAD to NADH is also the basis for the Emit assay of alcohol, but through the catalysis of ethanol to acetalde-hyde by alcohol dehydrogenase. Removing the LDH from the sample by ultrafiltration completely eliminates the interference.