Migrating epidermis produces AB2 collagen and requires continual collagen synthesis for movement
Migrating epidermis produces AB2 collagen and requires continual collagen synthesis for movement
复制标题
迁移表皮产生AB2胶原蛋白，需要持续合成胶原蛋白才能运动
作者：
K. Stenn;Joseph A. Madri;F. Roll
上皮细胞在体内伤口愈合、器官形成和肿瘤侵袭过程中表现出显著的运动性1。我们一直对上皮运动的控制感兴趣。从早期的研究中，我们知道，在体外上皮运动需要持续存在的血清component 2，3和适当的substratum 4。在培养分离的上皮细胞时，已发现胶原包被的培养皿比无包被的表面提供更好的基质5。由于其他实验室的研究表明上皮产生胶原6 -8，因此研究胶原在上皮运动中的作用似乎是合适的。本文报道了一个明确的皮肤外植体组织培养系统的形态学和代谢研究，包括上皮生长和表皮。观察结果支持的可能性，胶原蛋白的持续合成是必要的上皮细胞的运动。
EPITHELIAL CELLS demonstrate remarkable motility during the in vivo processes of wound healing, organogenesis and neoplastic invasion1. We have been interested in the control of epithelial motility. From earlier studies we know that in vitro epithelial movement requires the continual presence of a serum component2,3 and a proper substratum4. In culturing dissociated epithelial cells, collagen-coated dishes have been found to provide a better substratum than non-coated surfaces5. As studies in other laboratories had suggested that epithelium produces collagen6–8, it seemed appropriate to investigate the role of collagen in epithelial movement. Reported here are morphological and metabolic studies of a well defined tissue culture system of skin explants involving both epithelial outgrowth and epiboly. The observations support the possibility that the continual synthesis of collagen is necessary for epithelial cell movement.