Splicing segregation:: The minor spliceosome acts outside the nucleus and controls cell proliferation
Splicing segregation:: The minor spliceosome acts outside the nucleus and controls cell proliferation
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DOI：
10.1016/j.cell.2007.09.043
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发表时间：
2007-11-16
期刊：
影响因子：
64.5
通讯作者：
Mueller, Ferenc
中科院分区：
文献类型：
--
作者：
Koenig, Harald;Matter, Nathalie;Mueller, Ferenc
真核生物中两个平行mRNA剪接系统的功能相关性和进化-丰度和剪接速率不同的主要和次要剪接体-知之甚少。我们在这里报告，部分剪接前mRNA含有次要类内含子进行核出口和次要类snRNAs主要是在脊椎动物的细胞质。与次要剪接体的细胞质干扰进一步表明其功能与细胞核分离。与此相一致的是，在有丝分裂过程中，小的剪接只受到微弱的影响。通过选择性干扰斑马鱼发育和哺乳动物细胞中的snRNA功能，我们揭示了细胞周期进程中小剪接的保守作用。我们认为，分离的剪接系统允许加工部分未剪接的细胞质转录本，出现不同的剪接率的结果。分离提供了一种机制，占剪接体进化在一个单一的血统，并提供了一种手段，核独立控制基因表达。
The functional relevance and the evolution of two parallel mRNA splicing systems in eukaryotes - a major and minor spliceosome that differ in abundance and splicing rate - are poorly understood. We report here that partially spliced pre-mRNAs containing minor-class introns undergo nuclear export and that minor-class snRNAs are predominantly cytoplasmic in vertebrates. Cytoplasmic interference with the minor spliceosome further indicated its functional segregation from the nucleus. In keeping with this, minor splicing was only weakly affected during mitosis. By selectively interfering with snRNA function in zebrafish development and in mammalian cells, we revealed a conserved role for minor splicing in cell-cycle progression. We argue that the segregation of the splicing systems allows for processing of partially unspliced cytoplasmic transcripts, emerging as a result of different splicing rates. The segregation offers a mechanism accounting for spliceosome evolution in a single lineage and provides a means for nucleus-independent control of gene expression.