ROLE OF THE FLOOR PLATE IN AXONAL PATTERNING IN THE ZEBRAFISH CNS
ROLE OF THE FLOOR PLATE IN AXONAL PATTERNING IN THE ZEBRAFISH CNS
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DOI：
10.1016/0896-6273(92)90027-b
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发表时间：
1992-10-01
期刊：
影响因子：
16.2
通讯作者：
HATTA, K
中科院分区：
文献类型：
--
作者：
HATTA, K
为了确定底板（FP）在中枢神经系统发育中的作用，我使用了标记技术，包括免疫标记，分析剑水蚤突变胚胎，缺乏FP。除了前脑，突变表型几乎完全局限于腹侧中枢神经系统中线附近。在中脑，腹侧神经元的数量减少，细胞模式受到干扰。相反，脊髓中的神经元排列几乎是正常的，特别是包括初级和次级运动神经元。脑和脊髓中的纵向轴突束都是紊乱的。激光消融FP在野生型胚胎局部phenocopies剑水蚤轴突障碍，和野生型FP前体细胞移植到突变体局部拯救的干扰。这些结果表明FP在原位轴突的寻路和成束中具有重要作用，特别是在其纵向过程中。
To determine the role of the floor plate (FP) in CNS development, I have used labeling techniques, including immunolabeling, to analyze cyclops mutant embryos, which lack the FP. Except for the anterior brain, the mutant phenotype is almost exclusively confined to the vicinity of the ventral CNS midline. In the midbrain, the number of ventral neurons is reduced and cell patterning is disturbed. In contrast, the neuronal arrangement in the spinal cord is almost normal, including in particular both primary and secondary motoneurons. Longitudinal axonal bundles are disorganized in both the brain and spinal cord. Laser ablating the FP in wild-type embryos locally phenocopies cyclops axonal disturbances, and transplanting wild-type FP precursor cells into mutants locally rescues the disturbances. These results demonstrate a significant role for the FP in pathfinding and fasciculation by axons in situ, especially during their longitudinal courses.