Determination of antiidiotypic antibodies to anti-HLA IgG following blood transfusions.
Determination of antiidiotypic antibodies to anti-HLA IgG following blood transfusions.
复制标题
输血后抗 HLA IgG 的抗独特型抗体的测定。
DOI：
10.1097/00007890-198707000-00008
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发表时间：
1987
期刊：
影响因子：
6.2
通讯作者：
Jordan,SC
中科院分区：
文献类型：
--
作者：
Barkley,SC;Sakai,RS;Ettenger,RB;Fine,RN;Jordan,SC
已知在肾同种异体移植前进行输血（BT）可提高同种异体移植物存活率。这种效应的一种提出的机制是通过诱导抗独特型抗体来修饰对随后呈递的移植物抗原的免疫应答。为了确定BT后是否产生抗独特型抗体，我们研究了BT后患者血清中是否存在抗HLA I类IgG（即抗-抗-HLA）的抗独特型抗体。抗HLA抗独特型通过抗HLA抗体的酶免疫测定（EIA）的独特型特异性抑制来证明。用这种抑制技术，16例BT后患者中有8例的测试血清显示抗HLA靶血清的显著抑制（P< 0.05）。正常人血清对靶血清无抑制作用，试验血清对其它抗原抗体系统无抑制作用。试验血清的蛋白A吸收消除了抑制作用，表明抗独特型活性仅限于血清的IgG部分。BT后患者的血清抑制了抗HLAIgG的F（ab '）2片段的EIA结合活性，但正常志愿者的血清则不抑制。对从BT后患者连续获得的血清的研究表明，抗HLA抗独特型水平随时间和输血状态而波动。这些研究表明，抗HLA抗独特型抗体产生后BT。它们在介导移植前BT的已知同种异体移植物增强效应方面的重要性仍有待澄清。
Blood transfusions (BT) administered prior to renal allograft transplantation are known to enhance allograft survival. One proposed mechanism for this effect is through the induction of antiidiotypic antibodies that modify the immune response to subsequently presented graft antigens. In an attempt to determine if antiidiotypic antibodies are generated post-BT, we studied sera from post-BT patients for the presence of antiidiotypic antibodies to anti-HLA class I IgG (ie, anti-anti-HLA). Anti-HLA antiidiotypes were demonstrated by idiotype-specific inhibition of an enzyme immunoassay (EIA) for anti-HLA antibodies. Using this inhibition technique, test sera from 8 of 16 post-BT patients showed significant inhibition of the anti-HLA target sera (P< 0.05). Sera from normal individuals showed no inhibition of the target sera, and test sera did not inhibit other antigen-antibody systems. Protein A absorption of test sera eliminated the inhibitory effect, suggesting that the antiidiotypic activity was limited to the IgG fraction of the sera. The EIA binding activity of F (ab ‘) 2 fragments of anti-HLA IgG was suppressed by sera from patients post-BT but not by sera from normal volunteers. Studies of serially obtained sera from a post-BT patient showed that anti-HLA antiidiotype levels fluctuate with time and transfusion status. These studies demonstrate that anti-HLA antiidiotypic antibodies are generated post-BT. Their importance in mediating the known allograft enhancing effect of pretransplant BT remains to be clarified.