AREA CHANGES IN SLICES OF RAT-BRAIN DURING PREPARATION FOR HISTOLOGY OR ELECTRON-MICROSCOPY
AREA CHANGES IN SLICES OF RAT-BRAIN DURING PREPARATION FOR HISTOLOGY OR ELECTRON-MICROSCOPY
复制标题
DOI：
10.1111/j.1365-2818.1978.tb00117.x
复制
发表时间：
1978-01-01
影响因子：
2
通讯作者：
DEUTSCH, K
中科院分区：
文献类型：
--
作者：
HILLMAN, H;DEUTSCH, K
用2-3 mm或0.27mm厚的大鼠脑切片研究包埋和冷冻的效果。将6 μ m厚的石蜡切片封固并用苏木精和伊红或Marvinet al. 's（1954）银染色，并在每一步检查它们的面积。石蜡包埋2-3 mm厚的切片或Epon包埋0.27mm厚的切片，可使其面积缩小20- 30%。石蜡切片染色未改变其面积。单独使用浓度为15%的甘油对面积没有影响，但浓度为30%时，面积减少约20%。甘油处理过的0.27 mm厚的切片，当它们被转移到液氮或异戊烷中时，面积也会减小，但用氟利昂12代替甘油后，面积会增加。包埋或冷冻脑切片会引起其区域的变化，但包埋、切片和封片后的切片染色则不会。
Cerebral slices cut from rat brain, either 2-3 mm or 0.27 mm thick, were used to study the effect of embedding and freezing. Paraffin wax sections 6 .mu.m thick were mounted and stained with hematoxylin and eosin or Marsland et al.''s (1954) silver stain, and their areas were examined at each step. Embedding in paraffin wax of slices 2-3 mm thick, or in Epon of slices 0.27 mm thick, caused a diminution of their areas by 20-30%. Staining of paraffin wax sections did not alter their areas. Glycerol alone at 15% concentration had no effect on the areas, but at 30% concentration they were diminished by approximately 20%. Diminution of the areas of glycerol treated slices 0.27 mm thick also occurred when they were transferred to liquid N2 or to isopentane, but the areas increased after glycerol was replaced by Freon 12. Embedding or freezing cerebral slices caused changes in their areas, but the staining of sections after they had been embedded, sectioned and mounted did not.