The human CYP2C locus:: A prototype for intergenic and exon repetition splicing events
The human CYP2C locus:: A prototype for intergenic and exon repetition splicing events
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DOI：
10.1006/geno.1999.6063
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发表时间：
2000-02-01
期刊：
影响因子：
4.4
通讯作者：
Zaphiropoulos, PG
中科院分区：
文献类型：
--
作者：
Finta, C;Zaphiropoulos, PG
在人类中，有四个已知的 CYP2C 基因已被定位到染色体 10q24，顺序为 Cen-2C18-2C19-2C9-2C8-Tel。之前我们已经证明，连接邻近 2C18 和 2C19 基因外显子的剪接事件发生在人类肝脏和表皮中。这里提供的证据表明该簇的末端基因2C18和2C8也参与基因间剪接。最有趣的是，其中一些 2C18/2C8 RNA 由所有九个外显子组成，因此可以想象具有编码功能蛋白的潜力。此外，还检测到由不仅源自CYP2C8和CYP2C18基因而且源自CYP2C19基因的外显子组成的嵌合RNA种类。在所有情况下，来自不同 CYP2C 基因的外显子都连接在正确的规范剪接位点。然而，紧密相连的 RBP4 基因不参与与 CYP2C 基因的基因间剪接。此外，还发现 CYP2C8 基因表达会产生多种乱序 RNA 分子，包括含有某些外显子重复的物种。 (C) 2000 年学术出版社。
In human there are four known CYP2C genes that have been mapped to chromosome 10q24 with the order Cen-2C18-2C19-2C9-2C8-Tel. Previously we have shown that splicing events joining exons from the neighboring 2C18 and 2C19 genes occur in human liver and epidermis. Here evidence is presented that the terminal genes of this cluster, 2C18 and 2C8, are also involved in intergenic splicing. Most interestingly, several of these 2C18/2C8 RNAs were composed of all nine exons, thus conceivably having the potential for coding functional proteins. Moreover, chimeric RNA species consisting of exons originating not only from the CYP2C8 and CYP2C18 genes, but also from the CYP2C19 gene were detected. In all cases the exons from the different CYP2C genes were joined at the correct canonical splice sites. However, the closely linked RBP4 gene is not participating in intergenic splicing with the CYP2C genes. In addition, CYP2C8 gene expression was found to generate a variety of scrambled RNA molecules including species that contained repetitions of certain exons. (C) 2000 Academic Press.