Protein interaction mapping in C-elegans using proteins involved in vulval development
Protein interaction mapping in C-elegans using proteins involved in vulval development
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DOI：
10.1126/science.287.5450.116
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发表时间：
2000-01-07
期刊：
影响因子：
56.9
通讯作者：
Vidal, M
中科院分区：
文献类型：
--
作者：
Walhout, AJM;Sordella, R;Vidal, M
蛋白质相互作用映射使用大规模的双杂交分析已被提出作为一种方式来功能注释大量的未知蛋白质预测的完整的基因组序列。这种方法在秀丽隐杆线虫中进行了研究，从参与外阴发育的27种蛋白质开始。由此产生的地图揭示了已知的和新的潜在的相互作用，并提供了约100个未知的基因产物的功能注释。蛋白质相互作用绘图项目现在对于C来说是可行的。在全基因组范围内研究秀丽隐杆线虫，有助于理解这种生物体和人类疾病的分子机制。
Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans, starting with 27 proteins involved in vulval development. The resulting map reveals both known and new potential interactions and provides a functional annotation for approximately 100 uncharacterized gene products. A protein interaction mapping project is now feasible for C. elegans on a genome-wide scale and should contribute to the understanding of molecular mechanisms in this organism and in human diseases.