Brain pH has a significant impact on human postmortem hippocampal gene expression profiles
Brain pH has a significant impact on human postmortem hippocampal gene expression profiles
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DOI：
10.1016/j.brainres.2006.05.043
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发表时间：
2006-08-23
期刊：
影响因子：
2.9
通讯作者：
Leonard, S.
中科院分区：
文献类型：
--
作者：
Mexal, S.;Berger, R.;Leonard, S.
包括精神分裂症在内的脑神经生物学疾病的研究依赖于使用死后脑组织，其中了解各种生前和死后变量对基因表达的影响至关重要。在几个不同的大脑区域，pH值已被证明对死后大脑基因表达模式有很大的影响。在这些研究中尚未评估的一个区域是海马体，这是一个经常与精神分裂症研究有关的区域。在本研究中，我们表明，死后大脑pH值是相似的不同的大脑区域。脑pH值占海马基因表达谱的变化比任何其他参数评估，包括性别和精神分裂症。在一个独立的样本集的脑pH值的预测值也大于疾病，表明pH值代表了人类死后的基因表达研究精神分裂症的最重要的控制参数之一。(c)2006 Elsevier B.V.保留所有权利。
Studies of neurobiological disorders in the brain, including schizophrenia, rely on the use of postmortem brain tissues, in which an understanding of the effects of various pre- and postmortem variables on gene expression is critical. In several different brain regions, pH has been shown to have a large effect on postmortem brain gene expression patterns. One region that has not yet been evaluated in such studies is the hippocampus, a region often implicated in schizophrenia research. In the present study, we show that postmortem brain pH is similar across different brain regions. Brain pH accounted for greater variation in hippocampal gene expression profiles than any other parameter evaluated, including gender and schizophrenia. The predictive value of brain pH in an independent sample set was also greater than the disease, demonstrating that pH represents one of the most important control parameters in human postmortem gene expression studies in schizophrenia. (c) 2006 Elsevier B.V. All rights reserved.