INDUCTION OF LOW-DENSITY AND UP-REGULATION OF CD11B EXPRESSION OF NEUTROPHILS AND EOSINOPHILS BY DEXTRAN SEDIMENTATION AND CENTRIFUGATION
INDUCTION OF LOW-DENSITY AND UP-REGULATION OF CD11B EXPRESSION OF NEUTROPHILS AND EOSINOPHILS BY DEXTRAN SEDIMENTATION AND CENTRIFUGATION
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DOI：
10.1016/0022-1759(94)90087-6
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发表时间：
1994-01-03
影响因子：
2.2
通讯作者：
KAUFFMAN, HF
中科院分区：
文献类型：
--
作者：
BERENDS, C;DIJKHUIZEN, B;KAUFFMAN, HF
以活化状态循环的中性粒细胞和嗜酸性粒细胞密度低。然而，纯化程序如葡聚糖沉降和离心可能会影响细胞的密度和功能。在本研究中，我们评估了葡聚糖沉降和随后的离心对中性粒细胞和嗜酸性粒细胞的密度和CD 11b表达的影响。全血直接密度分离导致17.7 +/- 9.0%低密度中性粒细胞（
Neutrophils and eosinophils circulating in an activated state are of low density. However, purification procedures such as dextran sedimentation and centrifugation may influence the density and function of cells. In the present study we have evaluated the effect of dextran sedimentation and subsequent centrifugation on the density and CD11b expression of neutrophils and eosinophils. Direct density separation of whole blood resulted in 17.7 +/- 9.0% low density neutrophils (