Attenuating Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on Vascular Smooth Muscle Cell Phenotype Changes to Osteoblastic Cells and Vascular Calcification Induced by High Fat Diet.
Attenuating Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on Vascular Smooth Muscle Cell Phenotype Changes to Osteoblastic Cells and Vascular Calcification Induced by High Fat Diet.
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DOI：
10.3390/nu12092777
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发表时间：
2020-09-11
期刊：
影响因子：
5.9
通讯作者：
Byun K
中科院分区：
文献类型：
--
作者：
Son M;Oh S;Jang JT;Park CH;Son KH;Byun K
晚期糖基化终产物/AGEs受体（AGEs/RAGE）或Toll样受体4（TLR 4）诱导成骨样细胞中的血管平滑肌细胞（VSMC）表型改变和血管钙化。我们分析了Ecklonia cava提取物（ECE）或邻苯三酚-间苯三酚-6，6-bieckol（PPB）对高脂饮食（HFD）引起的VSMC表型变化和血管钙化的影响。HFD未调节小鼠主动脉中的活化B细胞（NF-κB）的TGF β、TLR 4、转化生长因子β（TGFβ）、骨形态发生蛋白2（BMP 2）、蛋白激酶C（PKC）和核因子κ轻链增强子信号。ECE和PPB恢复了这些信号通路的增加。AGE或棕榈酸酯处理的VSMC显示与动物相似的变化。HFD增加成骨细胞样VSMC，通过测量主动脉中的核心结合因子α-1（CBFα-1）和骨钙素表达以及碱性磷酸酶（ALP）活性来评估。通过钙沉积率分析，ECE和PPB可减少血管钙化，HFD可增加茜素红S染色。PPB和ECE降低收缩压、舒张压和平均血压，而HFD使收缩压、舒张压和平均血压升高。PPB和ECE可减少VSMC向成骨样细胞的表型转变和血管钙化，从而降低血压。
Advanced glycation end products/receptor for AGEs (AGEs/RAGEs) or Toll like receptor 4 (TLR4) induce vascular smooth muscle cell (VSMC) phenotype changes in osteoblast-like cells and vascular calcification. We analyzed the effect of Ecklonia cava extract (ECE) or pyrogallol-phloroglucinol-6,6-bieckol (PPB) on VSMC phenotype changes and vascular calcification prompted by a high-fat diet (HFD). HFD unregulated RAGE, TLR4, transforming growth factor beta (TGFβ), bone morphogenetic protein 2 (BMP2), protein kinase C (PKC), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signals in the aorta of mice. ECE and PPB restored the increase of those signal pathways. AGE- or palmitate-treated VSMC indicated similar changes with the animal. HFD increased osteoblast-like VSMC, which was evaluated by measuring core-binding factor alpha-1 (CBFα-1) and osteocalcin expression and alkaline phosphatase (ALP) activity in the aorta. ECE and PPB reduced vascular calcification, which was analyzed by the calcium deposition ratio, and Alizarin red S stain was increased by HFD. PPB and ECE reduced systolic, diastolic, and mean blood pressure, which increased by HFD. PPB and ECE reduced the phenotype changes of VSMC to osteoblast-like cells and vascular calcification and therefore lowered the blood pressure.
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影响因子：
5.3
作者：
Kameda T;Ohkawa R;Yano K;Usami Y;Miyazaki A;Matsuda K;Kawasaki K;Sugano M;Kubota T;Tozuka M
通讯作者：
Tozuka M
AGE-RAGE 信号生成特定的 NF-κB RelA“条形码”，指导 I 型胶原蛋白的表达。
影响因子：
4.6
作者：
Peng Y;Kim JM;Park HS;Yang A;Islam C;Lakatta EG;Lin L
通讯作者：
Lin L
影响因子：
6.1
作者：
Chen, Neal X.;Duan, Danxia;Moe, Sharon M.
通讯作者：
Moe, Sharon M.
影响因子：
3.2
作者：
Lee, Seung-Hong;Han, Ji-Sook;Jeon, You-Jin
通讯作者：
Jeon, You-Jin
影响因子：
4.8
作者：
Byon, Chang Hyun;Javed, Amjad;Chen, Yabing
通讯作者：
Chen, Yabing