Human cell mutagenicity of oxygenated, nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols
Human cell mutagenicity of oxygenated, nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols
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DOI：
10.1016/s0165-1218(96)90103-2
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发表时间：
1996-12-20
期刊：
影响因子：
--
通讯作者：
Crespi, CL
中科院分区：
文献类型：
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作者：
Durant, JL;Busby, WF;Crespi, CL
多环芳烃化合物（PAC）是城市空气中普遍存在的污染物，可能对人体健康构成威胁。为了更好地评估与这类化合物相关的健康风险，在基于人B淋巴母细胞的正向突变试验中检测了城市气溶胶样品中已确定（55）或疑似存在（12）的共67种PAC的致突变性。使用的细胞系（命名为hlAlv 2）组成型表达细胞色素P3501 A1，已知细胞色素P3501 A1是许多前诱变剂代谢所必需的。测试的PAC包括39种多环芳烃（PAH），19种含氧PAH（oxy-PAH）和9种NO2-取代的PAH（nitro-PAH）。共有26种PAH具有致突变性。在比较致突变PAH与苯并[a]芘（B[a]P）的最小致突变浓度时发现，二苯并[a，l]芘（DB[al]P）、环戊[c，d]芘（CPP）、萘并[2.1-a]芘、二苯并[a.e]芘（DB[ae]P）和1-甲基苯并[a]芘的最小致突变浓度分别为24 +/- 21、6.9 +/- 4.2、3.2 +/- 3.0、二苯并[a，k]荧蒽和B[a]P的致突变性分别为B[a]P的2.9 +/- 7.9和1.6 +/- 1.4倍，且二者的致突变性大致相同。其他19种致突变PAH的致突变性比B[a] P低2倍至1800倍。仅检测了非那酮的氧化PAH。7 H-苯并[d，e]蒽-7-酮、3-硝基-6 H-二苯并[B，d]吡喃-6-酮、环戊[c，d]芘-3（4 H）-酮、6 H-苯并[c，d]芘-6-酮（BPK）和蒽醌具有致突变性：然而，除了BPK之外，这些化合物的活性比B[a] P低50倍以上。BPK的活性比B[a] P低3倍。硝基蒽1-硝基芘、2-硝基荧蒽、3-硝基荧蒽、1，3-二硝基芘、1，6-二硝基芘（1.6-DNP）和1，8-二硝基芘。1.6-DNP是类似的4倍活性比B[a]P;其他6个致突变硝基多环芳烃之间的20和380倍活性比B[a] P。这些结果进行了讨论，确定在环境空气中最重要的诱变剂的相关性。根据环境气溶胶中PAC的报告浓度，CPP可能。在某些气溶胶中，DB[ae] P、DB[al]P和BPK的致突变性比B[a]P的致突变性大。
Polycyclic aromatic compounds (PAC) are ubiquitous pollutants in urban air that may pose risks to human health. In order to better assess the health risks associated with this class of compounds, a total of 67 PAC that either have been identified (55) or are suspected to be present (12) in urban aerosol samples were tested for mutagenicity in a forward mutation assay based on human B-lymphoblastoid cells. The cell line used (designated hlAlv2) constitutively expresses the cytochrome P3501Al, which is known to be necessary for the metabolism of many promutagens. The PAC tested included 39 polycyclic aromatic hydrocarbons (PAH), 19 oxygen-containing PAH (oxy-PAH) and nine NO2-substituted PAH (nitro-PAH). A total of 26 PAH were mutagenic. In comparing the minimum mutagenic concentrations of the mutagenic PAH with that of benzo[a]pyrene (B[a]P) it was found that dibenzo[a,l]pyrene (DB[al]P), cyclopenta[c,d]pyrene (CPP), naphtho[2.1-a]pyrene, dibenzo[a.e]pyrene (DB[ae]P) and 1-methylbenzo[a]pyrene were 24 +/- 21, 6.9 +/- 4.2, 3.2 +/- 3.0, 2.9 +/- 7.9 and 1.6 +/- 1.4, times, respectively, more mutagenic than B[a]P, and that dibenzo[a,k]fluoranthene and B[a]P were approximately equally mutagenic. The 19 other mutagenic PAH were between similar to 2 and similar to 1800 times less mutagenic than B[a]P. OF the oxy-PAH tested only phenalenone. 7H-benz[d,e]anthracen-7-one, 3-nitro-6H-dibenzo[b,d]pyran-6-one, cyclopenta[c,d]pyren-3(4H)-one, 6H-benzo[c,d]pyren-6-one (BPK) and anthanthrenequinone were mutagenic: however, with the exception of BPK, these were over 50 times less active than B[a]P. BPK was similar to 3 times less active than B[a]P. Seven of the nitro-PAH were mutagenic including 9-nitroanthracene 1-nitropyrene, 2-nitrofluoranthene, 3-nitrufluoranthene, 1,3-dinitropyrene, 1,6-dinitropyrene (1.6-DNP) and 1,8-dinitropyrene. 1.6-DNP was similar to 4 times less active than B[a]P; the six other mutagenic nitro-PAH were between 20 and 380 times less active than B[a]P. These results are discussed in terms of their relevance for determining the most important mutagens in ambient air. Based on reported concentrations of PAC in ambient aerosols, it is possible that CPP. DB[ae]P. DB[al]P and BPK could account for a greater proportion of the mutagenicity than B[a]P in some aerosols.