Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Gα13
Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Gα13
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DOI：
10.1126/science.280.5372.2112
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发表时间：
1998-06-26
期刊：
影响因子：
56.9
通讯作者：
Bollag, G
中科院分区：
文献类型：
--
作者：
Hart, MJ;Jiang, XJ;Bollag, G
将细胞外因子与单体鸟苷三磷酸酶（GTP酶）Rho的激活相联系的信号通路控制细胞骨架重排和细胞生长。异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）参与其中的一些通路，尽管它们的作用机制尚不清楚。两种G蛋白α亚基，Gα(12)和Gα(13)的GTP酶活性受到Rho鸟嘌呤核苷酸交换因子p115 RhoGEF的刺激。活化的Gα(13)与p115 RhoGEF紧密结合，并刺激其催化Rho上核苷酸交换的能力。相反，活化的Gα(12)抑制Gα(13)的刺激作用。因此，p115 RhoGEF能够直接将异源三聚体G蛋白α亚基与Rho的调节联系起来。
Signaling pathways that link extracellular factors to activation of the monomeric guanosine triphosphatase (GTPase) Rho control cytoskeletal rearrangements and cell growth. Heterotrimeric guanine nucleotide-binding proteins (G proteins) participate in several of these pathways, although their mechanisms are unclear. The GTPase activities of two G protein alpha subunits, G alpha(12 )and G alpha(13), are stimulated by the Rho guanine nucleotide exchange factor p115 RhoGEF. Activated G alpha(13) bound tightly to p115 RhoGEF and stimulated its capacity to catalyze nucleotide exchange on Rho, In contrast, activated G alpha(12) inhibited stimulation by G alpha(13). Thus, p115 RhoGEF can directly link heterotrimeric G protein a subunits to regulation of Rho.