Genome-Wide Analysis of Ethylene-Responsive Element Binding Factor-Associated Amphiphilic Repression Motif-Containing Transcriptional Regulators in Arabidopsis
Genome-Wide Analysis of Ethylene-Responsive Element Binding Factor-Associated Amphiphilic Repression Motif-Containing Transcriptional Regulators in Arabidopsis
复制标题
DOI：
10.1104/pp.109.151704
复制
发表时间：
2010-03-01
期刊：
影响因子：
7.4
通讯作者：
Rozwadowski, Kevin
中科院分区：
文献类型：
--
作者：
Kagale, Sateesh;Links, Matthew G.;Rozwadowski, Kevin
乙烯响应元件结合因子相关的两亲性抑制（EAR）基序是在乙烯反应性元件结合因子C2H2和成纤维蛋白/吲哚-3-乙酸的转录调节基序中鉴定的转录调节剂的转录调节基序。这些蛋白质的核心耳序位点的序列比较揭示了两个不同的保护模式：LXLXL和DLNXXP。包含这些基序的蛋白质通过负调节与发育，荷尔蒙和应力信号通路有关的基因进行负调节，在不同的生物学功能中起关键作用。通过全基因组的生物信息学分析，我们确定了拟南芥（拟南芥）中耳朵抑制组的完整曲目，其中包括219种属于21种不同的转录调节剂家族的蛋白质。这些蛋白质中约有72％含有LXLXL类型的EAR基序，22％包含DLNXXP类型的EAR基序，其余的6％具有LXLXL和DLNXXP重叠的基序。在体外和植物研究中发表的这些蛋白质中约有40％作为基因表达的负调节剂。耳序位点和毗邻区域的比较序列分析已经确定了可能影响EAR基序功能的其他首选残基和潜在的翻译后修饰位点。针对杨树（Populus trichocarpa），葡萄藤（Vitis Vinifera），大米（Oryza sativa）和高粱（高粱双色）的蛋白质数据库的同源搜索揭示了耳朵的图案在这些多样的植物物种中保存下来。这项全基因组分析是迄今为止对拟南芥中含耳朵基序的蛋白质的最广泛的调查，并提供了对其生物学作用的研究以及耳序介导的转录调控机制的研究。
The ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif is a transcriptional regulatory motif identified in members of the ethylene-responsive element binding factor, C2H2, and auxin/indole-3-acetic acid families of transcriptional regulators. Sequence comparison of the core EAR motif sites from these proteins revealed two distinct conservation patterns: LxLxL and DLNxxP. Proteins containing these motifs play key roles in diverse biological functions by negatively regulating genes involved in developmental, hormonal, and stress signaling pathways. Through a genome-wide bioinformatics analysis, we have identified the complete repertoire of the EAR repressome in Arabidopsis (Arabidopsis thaliana) comprising 219 proteins belonging to 21 different transcriptional regulator families. Approximately 72% of these proteins contain a LxLxL type of EAR motif, 22% contain a DLNxxP type of EAR motif, and the remaining 6% have a motif where LxLxL and DLNxxP are overlapping. Published in vitro and in planta investigations support approximately 40% of these proteins functioning as negative regulators of gene expression. Comparative sequence analysis of EAR motif sites and adjoining regions has identified additional preferred residues and potential posttranslational modification sites that may influence the functionality of the EAR motif. Homology searches against protein databases of poplar (Populus trichocarpa), grapevine (Vitis vinifera), rice (Oryza sativa), and sorghum (Sorghum bicolor) revealed that the EAR motif is conserved across these diverse plant species. This genome-wide analysis represents the most extensive survey of EAR motif-containing proteins in Arabidopsis to date and provides a resource enabling investigations into their biological roles and the mechanism of EAR motif-mediated transcriptional regulation.