Photoscan localization of GW-39 tumors in hamsters using radiolabeled anticarcinoembryonic antigen immunoglobulin G.
Photoscan localization of GW-39 tumors in hamsters using radiolabeled anticarcinoembryonic antigen immunoglobulin G.
复制标题
使用放射性标记的抗癌胚抗原免疫球蛋白 G 对仓鼠中的 GW-39 肿瘤进行光扫描定位。
作者：
David M. Goldenberg;Preston Df;F. Primus;Hans J. Hansen
对颊袋移植产生癌胚抗原（CEA）的人结肠癌GW-39的仓鼠进行了光扫描和器官放射性评估；人类肉瘤H.S.1；仓鼠无色素黑色素瘤，a . mel .3分别注射10 ~ 50 μCi 125i标记的异特异性抗cea免疫球蛋白G （IgG）或正常IgG。影像扫描显示肿瘤上的放射性吸收增加，并且经常在胸腔和膀胱区域，无论肿瘤或使用放射性标记的IgG制剂。即使是正常的仓鼠接受放射性标记制剂，胸部区域的放射性也会增加。然而，在携带小肿瘤的动物中证实了特定的肿瘤定位(注射放射性标记的抗cea IgG后，从仓鼠体内携带的GW-39肿瘤中恢复的放射性显示比从其他组织中恢复的放射性增加7.5至20倍。特异性或非特异性放射性标记制剂的摄取在另一种非cea产生的肿瘤中略有增加，在背景辐射中充分增加，可以通过光扫描看到肿瘤，特别是当肿瘤大且有血管时。显然，放射标记的非抗体成分异特异性IgG可以定位在某些肿瘤和正常组织的光扫描。然而，由于抗原抗体反应，肿瘤定位，正如我们在用抗cea抗体制剂治疗的产生cea的GW-39肿瘤中发现的那样，允许肿瘤的光扫描可视化，这些肿瘤太小而无法用放射性标记的正常IgG来证明。由此可见，CEA是一种适合放射抗体的肿瘤靶点。
Summary Photoscans and organ radioactivity were assessed in hamsters bearing cheek pouch grafts of a carcinoembryonic antigen (CEA)-producing human colonic carcinoma, GW-39; a human sarcoma, H.S.1; and a hamster amelanotic melanoma, A.Mel.3. The animals were given injections of 10 to 50 μCi 125I-labeled heterospecific anti-CEA immunoglobulin G (IgG) or normal IgG. The photoscans showed an increased uptake of radioactivity over the tumors and frequently over the areas of the thorax and urinary bladder, regardless of the tumor or the radiolabeled IgG preparation used. Even normal hamsters receiving either radiolabeled preparation showed an increased accretion of radioactivity over the thoracic region. A specific tumor localization, however, was demonstrated in animals bearing small ( The radioactivity recovered from GW-39 tumors borne in hamsters given injections of radiolabeled anti-CEA IgG revealed an increase of 7.5 to 20 times that recovered from other tissues. A slightly increased uptake of either the specific or nonspecific radiolabeled preparation was seen in the other, non-CEA-producing tumors studied, which was sufficiently increased over background radiation to permit visualization of the tumors by photoscanning, especially when the neoplasms were large and vascular. Evidently radiolabeled nonantibody components of heterospecific IgG can be localized in certain tumors and normal tissues by photoscanning. Nevertheless, tumor localization due to an antigen-antibody reaction, as we have found in CEA-producing GW-39 tumors treated with an anti-CEA antibody preparation, permits photoscan visualization of tumors too small to be demonstrated by radiolabeled normal IgG. It thus appears that CEA is a suitable tumor target for radioantibody by photoscanning.