Fibroblast Growth Factor Enhances Long‐term Potentiation in the Hippocampal Slice
Fibroblast Growth Factor Enhances Long‐term Potentiation in the Hippocampal Slice
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DOI：
10.1111/j.1460-9568.1990.tb00009.x
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发表时间：
1990-11
影响因子：
3.4
通讯作者：
H. Terlau;W. Seifert
中科院分区：
文献类型：
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作者：
H. Terlau;W. Seifert
最近，我们报道了用表皮生长因子（EGF）灌注海马脑片导致强直刺激后增强反应的增强。在本研究中，我们报告说，碱性成纤维细胞生长因子（FGF）也可以导致增强的反应。FGF是几种细胞类型的有丝分裂原，并对中枢神经系统（CNS）的神经元表现出神经营养作用。用浓度为10 - 9 M的FGF灌注大鼠海马切片。在CA 1区的细胞外和细胞内记录期间，如果使用单脉冲或成对脉冲刺激，则向灌注介质中添加FGF不会引起诱发反应的变化。此外，FGF对静息膜电位和输入电阻无影响。然而，在强直刺激后，FGF处理的切片与对照切片相比表现出增强幅度的增加。与EGF数据一起，这些结果支持这样的假设，即生长因子如对CNS神经元具有神经营养潜力的FGF可以影响突触功效。此外，这些结果表明，能够调节发育可塑性和再生可塑性的因素也可以调节突触可塑性。
Recently we reported that perfusion of hippocampal slices with epidermal growth factor (EGF) lead to enhancement of potentiated responses after tetanic stimulation. In the present study we report that basic fibroblast growth factor (FGF) can also lead to an enhancement of potentiated responses. FGF is a mitogen for several cell types and exhibits neurotrophic effects on neurons of the central nervous system (CNS). Rat hippocampal slices were perfused with FGF at a concentration of 10−9 M. During extra‐ and intracellular recordings in the CA1 ‐region, the addition of FGF to the perfusing medium produced no change in evoked responses if single pulse or paired pulse stimulation was used. Furthermore FGF had no influence on the resting membrane potential and input resistance. However, after tetanic stimulation, FGF‐treated slices showed an increase in the magnitude of potentiation compared to control slices. Taken together with the EGF data these results support the hypothesis that growth factors like FGF with neurotrophic potential on CNS‐neurons can influence synaptic efficacy. Furthermore these results show that factors which are able to modulate developmental plasticity and regenerative plasticity can also modulate synaptic plasticity.