Conventional transmission electron microscopy.
Conventional transmission electron microscopy.
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DOI：
10.1091/mbc.e12-12-0863
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发表时间：
2014-02
影响因子：
3.3
通讯作者：
Giddings TH Jr
中科院分区：
文献类型：
--
作者：
Winey M;Meehl JB;O'Toole ET;Giddings TH Jr
50 多年来，研究人员一直在使用透射电子显微镜 (TEM) 为细胞生物学做出贡献，并且 TEM 仍然是我们领域的一项重要技术。我们向新手简要介绍基于 TEM 的研究的组成部分，从通过样品成像进行样品制备开始。我们指出了 TEM 的局限性以及实验设计中需要考虑的问题。还列出了先进的电子显微镜技术。最后，我们为 TEM 的潜在新用户提供资源，以帮助启动他们的项目。
Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.