Isomer-specific antidiabetic properties of conjugated linoleic acid - Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression
Isomer-specific antidiabetic properties of conjugated linoleic acid - Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression
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DOI：
10.2337/diabetes.50.5.1149
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发表时间：
2001-05-01
期刊：
影响因子：
7.7
通讯作者：
Houseknecht, KL
中科院分区：
文献类型：
--
作者：
Ryder, JW;Portocarrero, CP;Houseknecht, KL
动物模型生物医学研究表明，共轭亚油酸 (CLA) 异构体具有许多有益的健康影响。此前，我们报道了 CLA 异构体混合物可改善 ZDF 大鼠的葡萄糖耐量，并在体外激活过氧化物酶体增殖物激活受体 (PPAR)-γ 反应元件。在这里，我们的目的是阐明特定 CLA 异构体对全身葡萄糖耐量、骨骼肌中胰岛素作用以及葡萄糖和脂质代谢中重要基因表达的影响。 ZDF大鼠喂食对照饮食（CON），两种CLA补充饮食之一（1.5％CLA），其中含有不同的CLA亚型（47％c9，t11；47.9％c10，t12，50：50；或91％c9，t11，c9，t11异构体），或配对喂养CON饮食以匹配50：50的摄入量。与所有其他 ZDF 治疗相比，50:50 饮食可减少肥胖并改善葡萄糖耐量。与所有其他治疗相比，骨骼肌中胰岛素刺激的葡萄糖转运和糖原合酶活性以 50:50 的比例得到改善。肌肉中的磷脂酰肌醇 3-激酶活性和 Akt 活性均未受到治疗的影响。与 ZDF 对照相比，肌肉和脂肪组织中的解偶联蛋白 2 被 c9、t11 和 50:50 上调。 c9、t11 和配对喂养的 ZDF 大鼠肝脏中 PPAR-gamma mRNA 下调。因此，50:50 大鼠的葡萄糖耐量改善至少部分归因于肌肉中胰岛素作用的改善，并且 CLA 效应不能简单地用食物摄入量减少来解释。
Conjugated linoleic acid (CLA) isomers have a number of beneficial health effects, as shown in biomedical studies with animal models. Previously, we reported that a mixture of CLA isomers improved glucose tolerance in ZDF rats and activated peroxisome proliferator-activated receptor (PPAR)-gamma response elements in vitro. Here, our aim was to elucidate the effect(s) of specific CLA isomers on whole-body glucose tolerance, insulin action in skeletal muscle, and expression of genes important in glucose and Lipid metabolism. ZDF rats were fed either a control diet (CON), one of two CLA supplemented diets (1.5% CLA) containing differing isoforms of CLA (47% c9,t11; 47.9% c10,t12, 50:50; or 91% c9,t11, c9,t11 isomers), or were pair-fed CON diet to match the intake of 50:50. The 50:50 diet reduced adiposity and improved glucose tolerance compared with all other ZDF treatments. Insulin-stimulated glucose transport and glycogen synthase activity in skeletal muscle were improved with 50:50 compared with all other treatments. Neither phosphatidlyinositol 3-kinase activity nor Akt activity in muscle was affected by treatment. Uncoupling protein 2 in muscle and adipose tissue was upregulated by c9,t11 and 50:50 compared with ZDF controls. PPAR-gamma mRNA was downregulated in liver of c9,t11 and pair-fed ZDF rats. Thus, the improved glucose tolerance in 50:50 rats is attributable to, at least in part, improved insulin action in muscle, and CLA effects cannot be explained simply by reduced food intake.