Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe
Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe
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DOI：
10.1016/j.febslet.2010.04.012
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发表时间：
2010-06-03
期刊：
影响因子：
3.5
通讯作者：
Kakinuma, Yoshimi
中科院分区：
文献类型：
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作者：
Chardwiriyapreecha, Soracom;Mukaiyama, Hiroyuki;Kakinuma, Yoshimi
我们鉴定了裂殖酵母的SPBC1685.07c作为一种新的液泡蛋白Avt5p，与酿酒酵母的液泡氨基酸转运蛋白Avt5p相似。Avt5p定位于液泡膜，并且在avt5破坏后，组氨酸、谷氨酸、酪氨酸、精氨酸、赖氨酸或丝氨酸的摄取受损。在氮饥饿期间，观察到的野生型细胞的液泡赖氨酸运输的瞬时增加仍然发生在突变体细胞，然而，摄取谷氨酸没有显着增加响应氮饥饿。我们的研究结果表明，在不同的生长条件下，Avt5p参与液泡运输的一组选择性的氨基酸。(C)2010年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
We identified SPBC1685.07c of Schizosaccharomyces pombe as a novel vacuolar protein, Avt5p, with similarity to vacuolar amino acid transporters Avt5p from Saccharomyces cerevisiae. Avt5p localizes to the vacuolar membrane and upon disruption of avt5, uptake of histidine, glutamate, tyrosine, arginine, lysine or serine was impaired. During nitrogen starvation, the transient increase of vacuolar lysine transport observed for wild-type cells still occurred in the mutant cells, however, uptake of glutamate did not significantly increase in response to nitrogen starvation. Our results show that under diverse growth conditions Avt5p is involved in vacuolar transport of a selective set of amino acids. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.