SPECIFIC RNA-BINDING BY AMINO-TERMINAL PEPTIDES OF ALFALFA MOSAIC-VIRUS COAT PROTEIN
SPECIFIC RNA-BINDING BY AMINO-TERMINAL PEPTIDES OF ALFALFA MOSAIC-VIRUS COAT PROTEIN
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DOI：
10.1002/j.1460-2075.1994.tb06312.x
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发表时间：
1994-02-01
期刊：
影响因子：
11.4
通讯作者：
GEHRKE, L
中科院分区：
文献类型：
--
作者：
BAER, ML;HOUSER, F;GEHRKE, L
特定的RNA -蛋白质相互作用和核糖核蛋白复合物对于许多生物过程是必不可少的，但是我们对核糖核蛋白颗粒如何形成和完成其生物学功能的理解是基本的。本文报道了苜蓿花叶病毒（AlMV）外壳蛋白或外壳肽与病毒RNA的相互作用。AlMV外壳蛋白是病毒颗粒形成和三种基因组RNA复制起始所必需的。我们研究了特异性RNA结合所需的蛋白质决定簇，并分析了复合物形成引起的潜在结构变化。结果表明，与公认的RNA结合基序缺乏一级序列同源性的病毒外壳蛋白的氨基末端，是必要的和足够的结合RNA。圆二色谱和电泳迁移率变动实验表明，RNA构象改变时，氨基末端的外壳蛋白肽结合到病毒RNA。肽-RNA相互作用在功能上是重要的，因为肽将在启动RNA复制中取代AlMV外壳蛋白。伴随RNA -肽复合物形成的表观构象变化可以产生一种结构，该结构不同于单独的病毒RNA，可以被病毒复制酶识别。
Specific RNA - protein interactions and ribonucleoprotein complexes are essential for many biological processes, but our understanding of how ribonucleoprotein particles form and accomplish their biological functions is rudimentary. This paper describes the interaction of alfalfa mosaic virus (AlMV) coat protein or peptides with viral RNA. AlMV coat protein is necessary both for virus particle formation and for the initiation of replication of the three genomic RNAs. We have examined protein determinants required for specific RNA binding and analyzed potential structural changes elicited by complex formation. The results indicate that the amino-terminus of the viral coat protein, which lacks primary sequence homology with recognized RNA binding motifs, is both necessary and sufficient for binding to RNA. Circular dichroism spectra and electrophoretic mobility shift experiments suggest that the RNA conformation is altered when amino-terminal coat protein peptides bind to the viral RNA. The peptide-RNA interaction is functionally significant because the peptides wilt substitute for AlMV coat protein in initiating RNA replication. The apparent conformational change that accompanies RNA - peptide complex formation may generate a structure which, unlike the viral RNA alone, can be recognized by the viral replicase.