Anti-Aging Potentials of Methylene Blue for Human Skin Longevity.
Anti-Aging Potentials of Methylene Blue for Human Skin Longevity.
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DOI：
10.1038/s41598-017-02419-3
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发表时间：
2017-05-30
影响因子：
4.6
通讯作者：
Cao K
中科院分区：
文献类型：
--
作者：
Xiong ZM;O'Donovan M;Sun L;Choi JY;Ren M;Cao K
氧化应激是皮肤老化的主要原因，包括皱纹，色素沉着和伤口愈合能力减弱。抗氧化剂在皮肤护理中的应用被公认为是延缓皮肤衰老过程的有效方法。亚甲基蓝（MB），一种传统的细胞靶向抗氧化剂，在培养的人皮肤成纤维细胞中显示出有效的ROS清除功效，这些细胞来自健康供体和患有早衰症（一种遗传性过早衰老疾病）的患者。与其他广泛使用的一般和靶向抗氧化剂相比，我们发现MB在刺激皮肤成纤维细胞增殖和延缓细胞衰老方面更有效。在体外重建的3D人体皮肤模型上进行的皮肤刺激试验表明，MB长期使用是安全的，即使在高浓度下也不会引起刺激。将MB应用于该3D皮肤模型进一步证明了MB改善了皮肤活力，促进了伤口愈合并增加了皮肤水合作用和真皮厚度。基因表达分析表明，MB治疗改变了皮肤中细胞外基质蛋白亚组的表达，包括弹性蛋白和胶原蛋白2A1的上调，这两种成分是健康皮肤的重要组成部分。总而言之，我们的研究表明MB在皮肤护理方面具有很大的潜力。
Oxidative stress is the major cause of skin aging that includes wrinkles, pigmentation, and weakened wound healing ability. Application of antioxidants in skin care is well accepted as an effective approach to delay the skin aging process. Methylene blue (MB), a traditional mitochondrial-targeting antioxidant, showed a potent ROS scavenging efficacy in cultured human skin fibroblasts derived from healthy donors and from patients with progeria, a genetic premature aging disease. In comparison with other widely used general and mitochondrial-targeting antioxidants, we found that MB was more effective in stimulating skin fibroblast proliferation and delaying cellular senescence. The skin irritation test, performed on an in vitro reconstructed 3D human skin model, indicated that MB was safe for long-term use, and did not cause irritation even at high concentrations. Application of MB to this 3D skin model further demonstrated that MB improved skin viability, promoted wound healing and increased skin hydration and dermis thickness. Gene expression analysis showed that MB treatment altered the expression of a subset of extracellular matrix proteins in the skin, including upregulation of elastin and collagen 2A1, two essential components for healthy skin. Altogether, our study suggests that MB has a great potential for skin care.