Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection.
Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection.
复制标题
牙鲆磷酸酶和张力蛋白同源物（PTEN）-miRNA的调控及其在自噬、凋亡和病原体感染中的作用
DOI：
10.3390/ijms21207725
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发表时间：
2020-10-19
影响因子：
5.6
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Li W;Guan X;Sun L
MicroRNAs (miRNAs) are small non-coding RNAs with important roles in diverse biological processes including immunity. Japanese flounder (Paralichthys olivaceus) is an aquaculture fish species susceptible to the infection of bacterial and viral pathogens including Edwardsiella tarda. In a previous study, pol-miR-novel_547, a novel miRNA of flounder with unknown function, was found to be induced by E. tarda. In the present study, we investigated the regulation and function of pol-miR-novel_547 and its target gene. We found that pol-miR-novel_547 was regulated differently by E. tarda and the viral pathogen megalocytivirus, and pol-miR-novel_547 repressed the expression of PTEN (phosphatase and tensin homolog) of flounder (PoPTEN). PoPTEN is ubiquitously expressed in multiple tissues of flounder and responded to bacterial and viral infections. Interference with PoPTEN expression in flounder cells directly or via pol-miR-novel_547 promoted E. tarda invasion. Consistently, in vivo knockdown of PoPTEN enhanced E. tarda dissemination in flounder tissues, whereas in vivo overexpression of PoPTEN attenuated E. tarda dissemination but facilitated megalocytivirus replication. Further in vitro and in vivo studies showed that PoPTEN affected autophagy activation via the AKT/mTOR pathway and also modulated the process of apoptosis. Together these results reveal for the first time a critical role of fish PTEN and its regulatory miRNA in pathogen infection, autophagy, and apoptosis.
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影响因子：
11.1
作者：
He C;Klionsky DJ
通讯作者：
Klionsky DJ
日本牙鲆 (Paralichthys olivaceus) 的 pol-miR-194a 抑制 I 型干扰素反应并促进迟缓爱德华氏菌感染
影响因子：
4.7
作者：
Guan, Xiao-lu;Zhang, Bao-cun;Sun, Li
通讯作者：
Sun, Li
影响因子：
4.8
作者：
Huang, Guochang;Redelman-Sidi, Gil;Jiang, Xuejun
通讯作者：
Jiang, Xuejun
影响因子：
3.7
作者：
Matsuzaki Y;Sakuma T;Yamamoto T;Saya H
通讯作者：
Saya H
MiR-21 通过 PTEN/Akt 通路抑制自噬来介导肝细胞癌细胞索拉非尼耐药。
影响因子：
--
作者：
He C;Dong X;Zhai B;Jiang X;Dong D;Li B;Jiang H;Xu S;Sun X
通讯作者：
Sun X