Lauric Acid Stimulates Ketone Body Production in the KT-5 Astrocyte Cell Line
Lauric Acid Stimulates Ketone Body Production in the KT-5 Astrocyte Cell Line
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DOI：
10.5650/jos.ess16069
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发表时间：
2016-08-01
影响因子：
1.5
通讯作者：
Terada, Shin
中科院分区：
文献类型：
--
作者：
Nonaka, Yudai;Takagi, Tetsuo;Terada, Shin
椰子油作为一种潜在的治疗阿尔茨海默病的方法最近引起了相当大的关注，因为它含有大量的中链脂肪酸(MCFAs)，它的摄入被认为可以刺激肝酮的生成，为葡萄糖代谢受损的大脑提供了一种替代能源。在这项研究中，我们首先重新评估了大鼠口服椰子油后血浆酮小体的反应。我们发现，椰子油引起的血酮体浓度的增加可以忽略不计，与高油酸葵花籽油注射后观察到的结果没有显著差异。相比之下，椰子油显著增加了血浆游离脂肪酸浓度和月桂酸的含量，月桂酸是椰子油中的主要MCFA。接下来，为了阐明月桂酸是否能够激活KT-5星形胶质细胞的酮体生成能力，我们用50和100 mU的月桂酸处理KT-5星形胶质细胞株4h，月桂酸处理的细胞培养上清液中总酮体浓度的增加幅度大于油酸，这表明月桂酸可以直接和有效地激活KT-5星形胶质细胞的酮体生成。这些结果表明，摄入椰子油可能通过直接激活星形胶质细胞中的酮类生成，从而为邻近的神经元提供燃料，从而改善大脑健康。
Coconut oil has recently attracted considerable attention as a potential Alzheimer's disease therapy because it contains large amounts of medium-chain fatty acids (MCFAs) and its consumption is thought to stimulate hepatic ketogenesis, supplying an alternative energy source for brains with impaired glucose metabolism. In this study, we first reevaluated the responses of plasma ketone bodies to oral administration of coconut oil to rats. We found that the coconut oil-induced increase in plasma ketone body concentration was negligible and did not significantly differ from that observed after high-oleic sunflower oil administration. In contrast, the administration of coconut oil substantially increased the plasma free fatty acid concentration and lauric acid content, which is the major MCFA in coconut oil. Next, to elucidate whether lauric acid can activate ketogenesis in astrocytes with the capacity to generate ketone bodies from fatty acids, we treated the KT-5 astrocyte cell line with 50 and 100 mu M lauric acid for 4 h. The lauric acid treatments increased the total ketone body concentration in the cell culture supernatant to a greater extent than oleic acid, suggesting that lauric acid can directly and potently activate ketogenesis in KT-5 astrocytes. These results suggest that coconut oil intake may improve brain health by directly activating ketogenesis in astrocytes and thereby by providing fuel to neighboring neurons.