An aptamer-based microfluidic device for thermally controlled affinity extraction
An aptamer-based microfluidic device for thermally controlled affinity extraction
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DOI：
10.1007/s10404-008-0322-4
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发表时间：
2009-04-01
影响因子：
2.8
通讯作者：
Lin, Qiao
中科院分区：
文献类型：
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作者：
Nguyen, ThaiHuu;Pei, Renjun;Lin, Qiao
我们提出了一种微流控装置的特定提取和热激活释放的分析物使用核酸适体。该装置主要由一个微室组成，该微室填充有作为固定相的适体功能化微珠，并与微加热器和温度传感器集成。我们通过提取代谢分析物（一磷酸腺苷与噻唑橙子偶联（TO-AMP））来演示设备操作，对RNA适体具有高选择性。然后使用芯片上热活化在低温下进行TO-AMP从适体表面的受控释放。这允许等度分析物洗脱，这消除了潜在的苛刻试剂的使用，并且当期望装置可重复使用时能够有效地再生适体表面。
We present a microfluidic device for specific extraction and thermally activated release of analytes using nucleic acid aptamers. The device primarily consists of a microchamber that is packed with aptamer-functionalized microbeads as a stationary phase, and integrated with a micro heater and temperature sensor. We demonstrate the device operation by performing the extraction of a metabolic analyte, adenosine monophosphate coupled with thiazole orange (TO-AMP), with high selectivity to an RNA aptamer. Controlled release of TO-AMP from the aptamer surface is then conducted at low temperatures using on-chip thermal activation. This allows isocratic analyte elution, which eliminates the use of potentially harsh reagents, and enables efficient regeneration of the aptamer surfaces when device reusability is desired.