Pharmacokinetic studies of a Chinese triple herbal drug formula
Pharmacokinetic studies of a Chinese triple herbal drug formula
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DOI：
10.1016/j.phymed.2008.01.006
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发表时间：
2008-11-01
期刊：
影响因子：
7.9
通讯作者：
Cao, H.
中科院分区：
文献类型：
--
作者：
Lv, H.;Sun, H.;Cao, H.
茵陈蒿汤制剂是临床常用的治疗肝病的经典中药方剂。本研究建立了一种快速、有效的高效液相色谱法（HPLC）同时测定大鼠血浆中6，7-二甲基七叶酮和京尼平苷的方法。在Dikma Diamonsil RP 18色谱柱（200 mm x 4.6 mm，5 μ m）上以乙腈-甲醇-水（0.1%甲酸）作为移动的相进行该测定，显示出可接受的线性、日内和日间精密度和准确度（R.S.D.）。= 5%），绝对回收率为74%，定量限为0.4和1.12 μ g/ml，检测限为0.06和0.09 μ g/ml。该方法成功地应用于研究复方配伍对YCHTP中6，7-二甲基七叶酮和京尼平苷的药代动力学的影响，当口服给药YCHTP的有效人体日剂量的大鼠。提示复方配伍对YCHTP的药代动力学有显著影响，并对YCHTP的配伍给药进行了阐述和验证。(C)2008年Elsevier GmbH。All rights reserved.
Yin Chen Hao Tang preparation (YCHTP) is a classic traditional Chinese medicine formula, which is commonly used for clinical treatment of hepatological diseases. In this study, a rapid and validated high-performance liquid chromatography (HPLC) method was developed to simultaneously identify 6,7-dimethylesculetin and geniposide in rat plasma. This assay was performed on a Dikma Diamonsil RP18 column (200mm x 4.6mm, 5 mu m) with acetonitrile-methanol-water (0.1% formic acid) as the mobile phase, showing acceptable linearity, intra- and inter-day precision and accuracy (R.S.D. = 5%), and absolute recovery for two analytes (74%); the limits of quantitation were 0.4 and 1.12 mu g/ml, and the limits of detection were 0.06 and 0.09 mu g/ml for two analytes. The developed method was successfully applied to study the effect of formula compatibility on the pharmacokinetics of 6,7-dimethylesculetin and geniposide in YCHTP when orally administrating an effective human daily dose of YCHTP to rats. We surmise that formula compatibility can significantly influence the pharmacokinetics of YCHTP, and we have elucidated and validated the compatible administration of YCHTP. (C) 2008 Elsevier GmbH. All rights reserved.