Relationship between HLA-DPA1 genetic polymorphism and anembryonic pregnancy
Relationship between HLA-DPA1 genetic polymorphism and anembryonic pregnancy
复制标题
HLA-DPA1基因多态性与无胚胎妊娠的关系
DOI：
10.1002/mgg3.1046
复制
发表时间：
2020
影响因子：
2
通讯作者：
Wang Li
中科院分区：
文献类型：
--
作者：
Wang Zhendong;Lu Xiaolin;Yao Xiuying;Liu Xinli;Zhao Linlin;Chang Shaoyan;Zhang Ting;Niu Bo;Wang Li
人类白细胞抗原（HLA）‐DP是HLA II类分子。HLA II类分子在胎盘滋养细胞中的过度表达可能导致妊娠丢失。然而，HLA - DP与妊娠丢失之间的关系尚不清楚。HLA - DPA1是一个HLA - DP肽链。本研究的目的是评估中国人群中enla - dpa1基因多态性与无胚妊娠（一种早期妊娠丢失）之间的关系。方法采用病例对照研究，比较无胚妊娠组和对照组hla - dpa1基因多态性的频率。纳入68例病例和122例对照。统计分析评估单核苷酸多态性（snp）与无胚胎妊娠易感性之间的相关性。采用MassARRAY高通量DNA分析方法分析19HLA - dpa1snp。为了探讨HLA - DPA1多态性对非胚胎妊娠的影响，采用ELISA法分析血清HLA - DPA1水平。结果rs1431403 （CC和TT）的同合子分型显著增加了病例组无胚妊娠的风险（ORCC= 3.13, 95% CI: 1.50 ~ 6.53； ORTT= 2.96, 95% CI: 1.31 ~ 6.66; ORCC+TT= 3.06, 95% CI: 1.62 ~ 5.78）。在HLA - DPA1高水平（≥1,500 pg/ml）的样本中，纯合子rs1431403基因型（nCC= 21, 43.8%; nTT= 20, 57.1%）比杂合子基因型更常见。结论hla - DPA1rs1431403可能是中国人群无胚胎妊娠的危险因素。纯合子rs1431403基因型（CC和TT）可能通过异常增加HLA - DPA1水平而增加无胚胎妊娠的风险。
BackgroundHuman leukocyte antigen (HLA)‐DP is an HLA class II molecule. Overexpression of HLA class II molecules in placental trophoblast cells may induce pregnancy loss. However, the association between HLA‐DP and pregnancy loss remains unclear. HLA‐DPA1 is an HLA‐DP peptide chain. The objective of this study was to assess the association betweenHLA‐DPA1genetic polymorphism and anembryonic pregnancy, a type of early pregnancy loss, in the Chinese population.MethodsA case–control study was designed to compare the frequencies ofHLA‐DPA1gene polymorphisms in an anembryonic pregnancy group and a control group. Sixty‐eight cases and 122 controls were recruited. Statistical analysis was performed to assess the correlation between single‐nucleotide polymorphisms (SNPs) and anembryonic pregnancy susceptibility. MassARRAY high‐throughput DNA analysis was used to analyze 19HLA‐DPA1SNPs. To explore howHLA‐DPA1polymorphism could affect anembryonic pregnancy, HLA‐DPA1 serum levels were analyzed by ELISA.ResultsHomozygous typing of rs1431403 (CC and TT) significantly increased the risk of anembryonic pregnancy in the case group (ORCC= 3.13, 95% CI: 1.50–6.53; ORTT= 2.96, 95% CI: 1.31–6.66; ORCC+TT= 3.06, 95% CI: 1.62–5.78). In samples with high HLA‐DPA1 levels (≥1,500 pg/ml), the homozygous rs1431403 genotypes (nCC= 21, 43.8%; nTT= 20, 57.1%) were observed more frequently than were heterozygous genotypes.ConclusionHLA‐DPA1rs1431403 may be a risk factor for anembryonic pregnancy in the Chinese population. Homozygous rs1431403 genotypes (CC and TT) may increase the risk of anembryonic pregnancy by aberrantly increasing the HLA‐DPA1 levels.