Non-linear stress-strain measurements of ex vivo produced oral mucosal equivalent (EVPOME) compared to normal oral mucosal and skin tissue.
Non-linear stress-strain measurements of ex vivo produced oral mucosal equivalent (EVPOME) compared to normal oral mucosal and skin tissue.
复制标题
与正常口腔粘膜和皮肤组织相比，对离体产生的口腔粘膜当量（EVPOME）进行非线性应力应变测量。
DOI：
10.1109/iembs.2011.6090075
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Hollman,KyleW
中科院分区：
文献类型：
--
作者：
Winterroth,Frank;Hollister,ScottJ;Feinberg,StephenE;Kuo,Shiuhyang;Fowlkes,JBrian;Ganguly,Arindam;Hollman,KyleW
采用直接力学试验法测定口腔粘膜组织的应力-应变曲线。对天然口腔粘膜组织和工程化装置进行测量，特别是临床开发的离体产生的口腔粘膜等效物（EVPOME）。当接种的细胞在EVPOME装置上增殖时，它们产生角化的保护性上层，其填充表面不规则性。这些转化可以进一步改变应力-应变参数，因为EVPOME中的细胞分化，与未接种的支架相比，更类似于天然口腔粘膜组织。除了在正常条件（37°C）下生长的组织装置之外，还在43°C下产生EVPOME。这些热应力样本模型可能的故障机制。结果从一个机械变形系统能够准确测量小（约1.0-1.5平方厘米）的圆柱形组织样本。通过将半径小于样品半径的圆形活塞降低到样品中心来产生变形。所产生的力用精密电子天平测量。培养的EVPOME的硬度低于AlloDerm®，但与天然猪颊组织相似。猪皮和猪上颚组织的硬度更低。正如预期的，热应激的EVPOME比正常培养的EVPOME硬度低，因为应激的角蛋白细胞被破坏，降低了组织的结构完整性。
Stress-strain curves of oral mucosal tissues were measured using direct mechanical testing. Measurements were conducted on both natural oral mucosal tissues and engineered devices, specifically a clinically developed ex vivo produced oral mucosal equivalent (EVPOME). As seeded cells proliferate on EVPOME devices, they produce a keratinized protective upper layer which fills in surface irregularities. These transformations can further alter stress-strain parameters as cells in EVPOME differentiate, more similar to natural oral mucosal tissues in contrast to an unseeded scaffold. In addition to tissue devices grown under normal conditions (37°C), EVPOMEs were also produced at 43°C. These thermally stressed specimens model possible failure mechanisms. Results from a mechanical deformation system capable of accurate measurements on small (approximately 1.0–1.5 cm2) cylindrical tissue samples are presented. Deformations are produced by lowering a circular piston, with a radius smaller than the sample radius, onto the center of the sample. Resulting force is measured with a precision electronic balance. Cultured EVPOME was less stiff than AlloDerm®, but similar to native porcine buccal tissue. Porcine skin and porcine palate tissues were even less stiff. Thermally stressed EVPOME was less stiff than normally cultured EVPOME as expected because stressed keratin cells were damaged reducing the structural integrity of the tissue.