Normalization of full-length enriched cDNA
Normalization of full-length enriched cDNA
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DOI：
10.1039/b715110c
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发表时间：
2008-01-01
影响因子：
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通讯作者：
Lukyanov, Sergey A.
中科院分区：
文献类型：
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作者：
Bogdanova, Ekaterina A.;Shagin, Dmitry A.;Lukyanov, Sergey A.
由于不同转录本在细胞和组织中的丰度有很大的差异，分析cDNA文库中的稀有信息是极其困难的。因此，对于罕见的转录本搜索和分析，均衡化(归一化)cDNA的生成是必不可少的。自1990年以来，人们已经发展了几种DNA归一化方法。这些方法中有许多已经被优化用于全长丰富的cDNA的标准化，并被用于各种应用，包括转录组分析和cDNA文库的功能筛选。其中一个过程(称为DSN标准化)是基于堪察加河蟹的双链特异核酸酶(DSN)的独特性质，允许产生具有高基因发现率的归一化cDNA文库。
Analysis of rare messages in cDNA libraries is extremely difficult due to the substantial variations in the abundance of different transcripts in cells and tissues. Therefore, for rare transcript searches and analyses, the generation of equalized (normalized) cDNA is essential. Several cDNA normalization methods have been developed since 1990. A number of these methods have been optimized for the normalization of full-length enriched cDNA, and used in various applications, including transcriptome analysis and functional screening of cDNA libraries. One such procedure (named DSN-normalization) is based on the unique properties of duplex-specific nuclease (DSN) from kamchatka crab and allows the generation of normalized cDNA libraries with a high gene discovery rate.