Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Type 1 Diabetes in Acute Pancreatitis Consortium.
Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Type 1 Diabetes in Acute Pancreatitis Consortium.
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DOI：
10.1097/mpa.0000000000002077
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发表时间：
2022-07-01
期刊：
影响因子：
2.9
通讯作者：
Conwell, Darwin L.
中科院分区：
文献类型：
--
作者：
Wasserfall, Clive;Dyer, Anne-Marie;Speake, Cate;Andersen, Dana K.;Baab, Kendall Thomas;Bellin, Melena D.;Broach, James R.;Campbell-Thompson, Martha;Chinchilli, Vernon M.;Lee, Peter J.;Park, Walter G.;Pratley, Richard E.;Saloman, Jami L.;Sims, Emily K.;Tang, Gong;Yadav, Dhiraj;Yazici, Cemal;Conwell, Darwin L.
关键词：
laboratory protocolpancreasimmunologytype 1 diabetesbiosamplesbiorepositoryAPacute pancreatitisCPDPCChronic Pancreatitis Diabetes Pancreas Cancer ConsortiumDCCdata coordinating centerDKCRNIDDK Central RepositoryDREAMDiabetes RElated to Acute pancreatitis and its MechanismsLNliquid nitrogenNIDDKNational Institute of Diabetes and Digestive and Kidney DiseasesPBMCperipheral blood mononuclear cellsSOPstandard operating proceduresSTsample tracking moduleT1DAPCType 1 Diabetes in Acute Pancreatitis Consortium
生物样本采集、处理和储存方法的差异可能会产生相当大的差异和错误。因此，标准操作程序的最佳实践对于成功发现、开发和验证疾病生物标志物至关重要。在这里，我们描述了急性胰腺炎联盟 (T1DAPC) 1 型糖尿病 DREAM（与急性胰腺炎相关的糖尿病及其机制）研究期间为生物样本采集制定的标准操作程序。值得注意的是，这些方案是根据先前联盟的经验以及具有免疫学、胰腺炎和糖尿病专业知识的工作组的意见，采用综合流程制定的。出版和采用一致的生物样本协议将为未来的研究提供信息，并允许更好地比较不同的代谢研究工作。
Differences in methods for biospecimen collection, processing, and storage can yield considerable variability and error. Therefore, best practices for standard operating procedures are critical for successful discovery, development, and validation of disease biomarkers. Here, we describe standard operating procedures developed for biospecimen collection during the DREAM (Diabetes RElated to Acute pancreatitis and its Mechanisms) Study within the Type 1 Diabetes in Acute Pancreatitis Consortium (T1DAPC). Notably these protocols were developed using an integrative process based on prior consortium experience and with input from working groups with expertise in immunology, pancreatitis and diabetes. Publication and adoption consistent biospecimen protocols will inform future studies and allow for better comparisons across different metabolic research efforts.