Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: Identification of the Slp homology domain
Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: Identification of the Slp homology domain
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DOI：
10.1006/bbrc.2001.4803
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发表时间：
2001-05-04
影响因子：
3.1
通讯作者：
Mikoshiba, K
中科院分区：
文献类型：
--
作者：
Fukuda, M;Saegusa, C;Mikoshiba, K
Slp1-3（突触结合蛋白样蛋白 1-3）是一个新的羧基末端型（C 型）串联 C2 蛋白家族，与其他 C 型串联 C2 蛋白（例如突触结合蛋白和 Doca 家族）相比，与 granuphilin-a/Slp-4 的 C2 结构域具有更高的序列相似性。然而，原始 Slp1-3 的氨基 (N) 末端结构域不包含任何已知的蛋白质基序，并且彼此之间不显示任何序列相似性。我们报告了 Slp2 的四种选择性剪接异构体（指定为 Slp2-a-d，原始 Slp2 更名为 Slp2-c）和 Slp3 的两种选择性剪接异构体（Slp3-a 和 Slp3-b，原始 Slp3）。这些亚型具有相同的C端串联C2结构，但由于不同外显子的交替使用，它们的N端核苷酸序列完全不同。 Slp1、Slp2-a、Slp3-a 和 Slp4 氨基末端结构域的序列比对揭示了 Sip 家族中存在两个保守区域，称为 SHD1（Slp 同源结构域 1）和 SHD2，它们可能充当蛋白质相互作用位点。 Slp3-a和Slp4的SHD1和SHD2被假定的Zn2+结合序列分开，而Slp1和Slp2缺乏这样的Zn2+结合序列并且它们的SHD1和SHD2连接在一起。此外，我们发现Slp2-a/c/d mRNA在不同的小鼠组织和不同的发育阶段分布有差异，表明这些转录本可能受到不同启动子的调节。 (C) 2001 年学术出版社。
Slp1-3 (synaptotagmin-like protein 1-3) is a new family of carboxyl-terminal-type (C-type) tandem C2 proteins that show higher sequence similarity to the C2 domains of granuphilin-a/Slp-4 than those of other C-type tandem C2 proteins (e.g., synaptotagmin and the Doca family). However, the amino (N)-terminal domains of the original Slp1-3 do not contain any known protein motifs and do not show any sequence similarities to each other. We report four alternative splicing isoforms of Slp2 (designated Slp2-a-d, with the original Slp2 renamed Slp2-c) and two alternative splicing isoforms of Slp3 (Slp3-a and Slp3-b, the original Slp3). These isoforms share the same C-terminal tandem C2 structures, but their N-terminal nucleotide sequences are completely different due to the alternate use of different exons. Sequence alignment of the Slp1, Slp2-a, Slp3-a, and Slp4 amino terminal domains reveals the presence of two conserved regions among the Sip family, designated SHD1 (Slp homology domain 1) and SHD2, which may function as protein interaction sites. The SHD1 and SHD2 of Slp3-a and Slp4 are separated by a putative Zn2+-binding sequence, whereas Slp1 and Slp2 lack such Zn2+-binding sequences and their SHD1 and SHD2 are linked together. In addition, we show that the Slp2-a/c/d mRNAs are differentially distributed in different mouse tissues and at different stages of development, suggesting that these transcripts may be regulated by different promoters. (C) 2001 Academic Press.