Influence of pore size on the redifferentiation potential of human articular chondrocytes in poly(urethane urea) scaffolds
Influence of pore size on the redifferentiation potential of human articular chondrocytes in poly(urethane urea) scaffolds
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DOI：
10.1002/term.350
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发表时间：
2011-07
影响因子：
3.3
通讯作者：
H. Stenhamre;U. Nannmark;A. Lindahl;P. Gatenholm;M. Brittberg
中科院分区：
文献类型：
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作者：
H. Stenhamre;U. Nannmark;A. Lindahl;P. Gatenholm;M. Brittberg
支架的化学和物理性质影响细胞行为，最终决定组织工程软骨结构的性能和结果。本研究的目的是评估可降解多孔聚氨酯脲支架是否可以作为软骨组织工程的合适材料。我们还研究了体外扩增的成人软骨细胞的扩增后再分化和软骨组织形成是否可以通过支架结构的受控修饰来调节。用软骨细胞接种具有不同孔径（< 150 μm、150-300 μm和300-500 μm）的支架，并在体外进行软骨形成和成骨诱导。具有较小孔径的聚（氨基甲酸酯脲）支架增强了透明样细胞外基质，从而促进了新软骨形成。相反，软骨细胞在具有较大孔径的支架中更大程度地分化成骨途径。总之，我们的研究结果表明，聚（氨基甲酸酯脲）可能是有用的软骨组织工程支架材料。此外，体外扩增的人关节软骨细胞的成软骨和成骨分化能力可以受到支架结构的影响。通过定制孔径，组织工程软骨结构的性能可能会受到影响，因此也会影响长期的临床结果。版权所有© 2010约翰威利父子有限公司.
The chemical and physical properties of scaffolds affect cellular behaviour, which ultimately determines the performance and outcome of tissue‐engineered cartilage constructs. The objective of this study was to assess whether a degradable porous poly(urethane urea) scaffold could be a suitable material for cartilage tissue engineering. We also investigated whether the post‐expansion redifferentiation and cartilage tissue formation of in vitro expanded adult human chondrocytes could be regulated by controlled modifications of the scaffold architecture. Scaffolds with different pore sizes, < 150 µm, 150–300 µm and 300–500 µm, were seeded with chondrocytes and subjected to chondrogenic and osteogenic induction in vitro. The poly(urethane urea) scaffold with the smaller pore size enhanced the hyaline‐like extracellular matrix and thus neocartilage formation. Conversely, the chondrocytes differentiated to a greater extent into the osteogenic pathway in the scaffold with the larger pore size. In conclusion, our results demonstrate that poly(urethane urea) may be useful as a scaffold material in cartilage tissue engineering. Furthermore, the chondrogenic and the osteogenic differentiation capacity of in vitro expanded human articular chondrocytes can be influenced by the scaffold architecture. By tailoring the pore sizes, the performance of the tissue‐engineered cartilage constructs might be influenced and thus also the clinical outcome in the long run. Copyright © 2010 John Wiley & Sons, Ltd.