QUANTITATIVE ELECTRON-MICROSCOPIC AUTORADIOGRAPHY OF INSULIN, GLUCAGON, AND SOMATOSTATIN BINDING-SITES ON ISLETS
QUANTITATIVE ELECTRON-MICROSCOPIC AUTORADIOGRAPHY OF INSULIN, GLUCAGON, AND SOMATOSTATIN BINDING-SITES ON ISLETS
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DOI：
10.1126/science.6126003
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
ORCI, L
中科院分区：
文献类型：
--
作者：
PATEL, YC;AMHERDT, M;ORCI, L
用[125I]胰岛素、[125I]胰高血糖素和[125I]酪氨酸生长抑素对大鼠胰岛进行单层培养后，在B、A、D细胞上发现了与每个放射性标记配体相关的特异性放射自显影颗粒。B、A和D细胞的标记密度与3种激素对每个胰岛细胞的已知作用有很好的相关性。
After monolayer cultures of rat islets were exposed to [125I]insulin, [125I]glucagon and [125I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A and D cells. The density of labeling of the B, A and D cells with each labeled ligand correlated well with the known actions of the 3 hormones on each of the islet cells.