LIFE SPAN OF INDIVIDUAL YEAST CELLS
LIFE SPAN OF INDIVIDUAL YEAST CELLS
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DOI：
10.1038/1831751a0
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发表时间：
1959-01-01
期刊：
影响因子：
64.8
通讯作者：
JOHNSTON, JR
中科院分区：
文献类型：
--
作者：
MORTIMER, RK;JOHNSTON, JR
酵母细胞通过芽殖分裂产生两个可识别的细胞，即产生芽的母细胞和从芽发育而来的子细胞。子细胞的细胞壁大概是新合成的，而母细胞的细胞壁则通过分裂保留了全部或至少部分的特性--这是线性遗传的一个例子。母细胞的细胞壁没有重新形成，这可以通过与每个萌芽事件相关的疤痕积累来证明。电子显微照片3 - 5显示瘢痕面积约为细胞表面积的1%。如果，如Barton所指出的，在疤痕部位从未形成芽，那么单个细胞的寿命可能会受到可用芽位耗尽的限制。巴顿观察到一个细胞出芽23次，但没有观察到出芽的停止。其他渐进的变化也可能限制单个细胞可以完成的分裂次数。本报告描述了一个实验，旨在确定寿命的单酵母细胞，包括考虑寿命限制的可能机制。
THE division of a yeast cell by budding gives rise to two cells which are identifiable, that is, the mother cell from which the bud arose, and the daughter cell which developed from the bud. The wall of the daughter cell presumably is synthesizedde novowhile that of the mother cell retains all or at least part of its identity through the division—an example of linear inheritance1. That the wall of the mother cell is not reformed is evidenced by the accumulation of scars associated with each budding event2. Electron micrographs3–5reveal a scar area that is approximately 1 per cent of the surface area of the cells. If, as indicated by Barton2, a bud is never formed at the site of a scar, then it is possible that the life-span of individual cells would be limited by depletion of available budding sites. Barton observed one cell to bud 23 times but did not observe a cessation of budding. Other progressive changes also might limit the number of divisions that a single cell can complete. This report describes an experiment designed to determine the life-span of single yeast cells and includes consideration of possible mechanisms for life-span limitation.