Usefulness of TA02 (napsin A) to distinguish primary lung adenocarcinoma from metastatic lung adenocarcinoma
Usefulness of TA02 (napsin A) to distinguish primary lung adenocarcinoma from metastatic lung adenocarcinoma
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DOI：
10.1016/s0169-5002(03)00194-6
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发表时间：
2003-08-01
期刊：
影响因子：
5.3
通讯作者：
Kato, H
中科院分区：
文献类型：
--
作者：
Hirano, T;Gong, YB;Kato, H
TA 02（napsin A）经双向凝胶电泳（2-DE）检测，分子量为35.0 kDa，等电点为5.29。我们开发了一种针对TA 02分子N-末端的小鼠单克隆抗体（克隆TMU-Ad 02（6A 1）），并试图研究其对周围肺结节性病变的免疫组化反应性，并评估其对原发性和转移性肺腺癌鉴别诊断的有用性。本文对109例肺癌（原发性肺癌76例，转移性肺腺癌33例）、5例间皮瘤、4例非典型腺瘤样增生（AAH）和5例肺结核瘤的手术切除标本进行了TA 02免疫组化检测。在43例原发性肺腺癌中，39例（90.7%）观察到阳性免疫组化反应与颗粒状胞浆染色模式。尽管7例大细胞癌中有2例（28.6%）呈颗粒状阳性，但免疫组化染色强度较弱。其他癌性病变包括转移性肺腺癌和间皮瘤均为完全阴性。与TA 02和SpA的免疫组化反应性相比，TA 02在阳性率和免疫组化强度方面均上级SpA。结论：TA 02在肺肿瘤中的表达对鉴别原发性肺腺癌和转移性肺腺癌有重要价值，其鉴别诊断价值上级于SpA。(C)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
TA02 (napsin A) associated with primary lung adenocarcinoma detected by the two-dimensional polyacrylamide get electrophoresis (2-DE), has a molecular weight of 35.0 kDa and an isoetectric point 5.29. We developed a mouse monoclonal antibody against the N-terminal of the TA02 molecule (clone TMU-Ad02 (6A1)) and attempted to investigate its immunohistochemical reactivity for nodular lesions of the peripheral lung, and evaluate its usefulness for making a differential diagnosis between primary and metastatic lung adenocarcinomas. The TA02-immunohistochemical evaluation was carried out using surgically resected materials of 109 cases of Lung cancer (76 cases of primary lung cancer and 33 cases of metastatic lung adenocarcinoma from other organs), five cases with mesothelioma, four cases with atypical adenomatous hyperplasia (AAH) and five cases with tuberculoma of the lung. In 39 out of 43 (90.7%) primary lung adenocarcinomas, positive immunohistochemical reactivity with a granular cytoplasmic staining pattern was observed. Even though two out of seven (28.6%) large cell carcinomas showed positive results with a granular staining pattern, the immunohistochemical staining intensity was weak. The other cancerous lesions containing metastatic lung adenocarcinoma and mesothelioma were all completely negative. In comparison with the immunohistochemical reactivity of TA02 and SpA, TA02 was superior to SpA both in terms of positive rate and immunohistochemical intensity. We concluded that the evaluation of TA02 expression in lung tumorous lesions was very valuable for discriminating between primary and metastatic Lung adenocarcinoma, and TA02 was superior to SpA as a discriminating marker. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.