Use of the polymerase chain reaction to investigate the delimitation of two agronomically important species of Stylosanthes (Aubl.) Sw.
Use of the polymerase chain reaction to investigate the delimitation of two agronomically important species of Stylosanthes (Aubl.) Sw.
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DOI：
10.1080/03746609408684819
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Everley J. Glover;A. Gillies;R. Abbott
中科院分区：
文献类型：
--
作者：
Everley J. Glover;A. Gillies;R. Abbott
摘要关于柱花草的两种分类存在分歧，柱花草属包含许多重要的热带牧草豆类。有人认为，尽管在农艺性状上存在差异，但东半球的柱花草和新大陆的柱花草应该在形态上非常相似的基础上被简化为一个单一的物种。RAPDS是一种使用聚合酶链式反应(PCR)产生遗传指纹的方法，用于分析两个分类群内部和之间的遗传距离。根据这些数据生成的物候图表明它们之间存在一定程度的差异，尽管无法确定是在特定还是亚特定水平上。
Summary There is disagreement over the classification of two species of Stylosantlies, a genus which contains a number of important tropical pasture legumes. It has been argued that the Old World species Stylosanthes fruticosa and the New World Stylosanthes scabra should be reduced to a single species on the basis of their close morphological similarity, despite differences in agronomic characteristics. RAPDs, a method of producing genetic finger-prints using the polymerase chain reaction (PCR), was employed to analyse genetic distances within and between the two taxa. Phenograms generated from this data suggest a degree of differentiation between them, although whether at the specific or sub-specific level could not be determined.