An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma
An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma
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DOI：
10.1194/jlr.m700113-jlr200
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发表时间：
2007-08-01
影响因子：
6.5
通讯作者：
Dahlback, Bjorn
中科院分区：
文献类型：
--
作者：
Axler, Olof;Ahnstrom, Josefin;Dahlback, Bjorn
载脂蛋白M（apoM）是属于脂质运载蛋白超家族的188个氨基酸、25 kDa的蛋白质。虽然apoM主要与高密度脂蛋白相关，但在所有主要脂蛋白类别中均发现apoM。为了方便apoM的临床研究，我们建立了一种检测人血浆apoM的夹心ELISA法。该方法已被用于调查正常apoM的变化，并建立健康个体的参考值，通过测量598个样本从北欧参考区间项目生物银行和数据库（NOBIDA）生物银行。对于18-49岁的女性，apoM的参考范围为0.58-1.18 μ mol/l，而对于501岁的女性和男性，参考范围为0.61-1.30 μ mol/l。载脂蛋白M与NOBIDA数据库中26种常见临床化学分析物的相关性研究显示，载脂蛋白M与血浆总胆固醇（r = 0.52）、LDL和HDL胆固醇（r分别为0.43和0.36）显著正相关。与HDL/总胆固醇比值或体重指数无统计学显著相关性。总之，用于测量人血浆中apoM的夹心ELISA显示，健康个体中apoM浓度与总胆固醇强烈相关。
Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu mol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol ( r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.