Histidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
Histidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
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幽门螺杆菌富含组氨酸的蛋白 Hpn 在体外形成淀粉样原纤维并抑制胃上皮 AGS 细胞的增殖
DOI：
10.1016/j.bbamcr.2011.04.005
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发表时间：
2011-08-01
影响因子：
5.1
通讯作者：
Sun, Hongzhe
中科院分区：
文献类型：
--
作者：
Ge, Ruiguang;Sun, Xuesong;Sun, Hongzhe
幽门螺杆菌可引起多种胃病，如胃炎、消化性溃疡、胃癌和粘膜相关淋巴组织淋巴瘤。Hpn是一种富含组氨酸的蛋白质，在这种细菌中大量存在，并在生理相关条件下形成寡聚体。在本研究中，Hpn寡聚体被发现开发淀粉样纤维证实负染色转换电子显微镜，硫磺素T和刚果红结合试验。Hpn的淀粉样纤维通过将细胞周期阻滞在G2/M期来抑制胃上皮AGS细胞的增殖，这可能与线粒体生物能量学的破坏密切相关，如细胞内ATP水平和线粒体膜电位的显著耗竭所反映的。本文提供的数据有助于阐明H.幽门感染(C)2011 Elsevier B. V.保留所有权利。
Helicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. (C) 2011 Elsevier B.V. All rights reserved.