Measuring the surface area of aluminum hydroxide adjuvant
Measuring the surface area of aluminum hydroxide adjuvant
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DOI：
10.1002/jps.10166
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发表时间：
2002-07-01
影响因子：
3.8
通讯作者：
Hem, SL
中科院分区：
文献类型：
--
作者：
Johnston, CT;Wang, SL;Hem, SL
传统的测定表面积的方法，氮气吸附，需要在分析前将样品完全干燥。这项技术不适合氢氧化铝助剂，因为它是由亚微米纤维颗粒组成，完全除去水分后就不可逆地团聚在一起。在本研究中，用重量/傅里叶变换红外光谱(FTIR)法测定了一种商品氢氧化铝助剂的比表面积，并测定了其吸水容量。该技术不需要完全干燥佐剂。经5次重复测定，商品氢氧化铝佐剂的平均比表面积为514m(2)/g，95%可信区间为36m(2)/g。用X射线衍射图和谢雷尔方程计算了初晶尺寸。平均计算尺寸为4.5×2.2×10 nm。在此基础上，计算出市售氢氧化铝佐剂的平均比表面积为509m(2)/g，95%可信区间为30m(2)/g，两者的结果吻合较好，说明这两种方法均可用于氢氧化铝佐剂比表面积的测定。用两种方法测定的高比表面积是氢氧化铝助剂的一个重要性质，也是其固有的高蛋白质吸附能力的基础。(C)2002年Wiley-Liss，Inc.和美国药学协会。
The traditional method of determining surface area, nitrogen gas sorption, requires complete drying of the sample prior to analysis. This technique is not suitable for aluminum hydroxide adjuvant because it is composed of submicron, fibrous particles that agglomerate irreversibly upon complete removal of water. In this study, the surface area of a commercial aluminum hydroxide adjuvant was determined by a gravimetric/FTIR method that measures the water adsorption capacity. This technique does not require complete drying of the adjuvant. Five replicate determinations gave a mean surface area of 514 m(2)/g and a 95% confidence interval of 36 m(2)/g for a commercial aluminum hydroxide adjuvant. The X-ray diffraction pattern and the Scherrer equation were used to calculate the dimensions of the primary crystallites. The average calculated dimensions were 4.5 x 2.2 x 10 nm. Based on these dimensions, the mean calculated surface area of the commercial aluminum hydroxide adjuvant was 509 m(2)/g, and the 95% confidential interval was 30 m(2)/g. The close agreement between the two surface area values indicates that either method may be used to determine the surface area of aluminum hydroxide adjuvant. The high surface area, which was determined by two methods, is an important property of aluminum hydroxide adjuvants, and is the basis for the intrinsically high protein adsorption capacity. (C) 2002 Wiley-Liss, Inc. and the American Pharmaceutical Association.