Midinfrared surface-plasmon resonance: A novel biophysical tool for studying living cells
Midinfrared surface-plasmon resonance: A novel biophysical tool for studying living cells
复制标题
DOI：
10.1063/1.3116143
复制
发表时间：
2009-05-15
影响因子：
3.2
通讯作者：
Aroeti, B.
中科院分区：
文献类型：
--
作者：
Golosovsky, M.;Lirtsman, V.;Aroeti, B.
我们讨论基于傅里叶变换红外（FTIR）光谱测定的表面等离子共振（SPR）技术。我们探索了红外表面等离激元技术在水溶液中生物学研究中的潜力，并将其与在可见光范围内运行的传统表面等离激元技术进行了比较。我们证明 SPR 技术在红外范围内的灵敏度并不低，实际上甚至更高。我们展示了应用 FTIR-SPR 进行生物学研究的几个示例：(i) 监测溶液中的 D-葡萄糖浓度，以及 (ii) 测量悬浮液中红细胞对 D-葡萄糖的摄取。我们强调了红外 SPR 在研究活细胞培养物方面的优势，并展示了如何使用该技术来表征 (i) 胆固醇渗透到质膜和 (ii) 转铁蛋白诱导的网格蛋白介导的内吞作用。
We discuss the surface-plasmon resonance (SPR) technique based on Fourier transform infrared (FTIR) spectrometry. We explore the potential of the infrared surface plasmon technique for biological studies in aqueous solutions and compare it with the conventional surface plasmon technique operating in the visible range. We demonstrate that the sensitivity of the SPR technique in the infrared range is not lower and in fact is even higher. We show several examples of applying FTIR-SPR for biological studies: (i) monitoring D-glucose concentration in solution and (ii) measuring D-glucose uptake by erythrocytes in suspension. We emphasize the advantages of infrared SPR for studying living cell cultures and show how this technique can be used for characterization of (i) cholesterol penetration into plasma membrane and (ii) transferrin-induced clathrin-mediated endocytosis.