Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population
Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population
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DOI：
10.1152/ajplung.00169.2002
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发表时间：
2002-12-01
影响因子：
4.9
通讯作者：
Brody, SL
中科院分区：
文献类型：
--
作者：
You, YJ;Richer, EJ;Brody, SL
在发育和修复过程中，气道上皮细胞增殖和分化的高度调控程序常常在疾病中被破坏。这些过程已在小鼠模型中进行了研究；然而，很难在体内分离和鉴定上皮细胞特异性反应。为了在体外研究这些过程，我们描述了小鼠气管上皮细胞原代培养模型的特征。以低密度（7.5 x 10(4) 细胞/cm(2)）接种的少量细胞迅速增殖并极化。随后，补充的培养基和气液界面条件导致高度分化的上皮细胞的发育，该上皮细胞由纤毛细胞和非纤毛细胞组成，具有天然气道的基因表达特征。基因改变或损伤的小鼠气管上皮细胞也反映了气管上皮细胞基因表达的体内模式。细胞传代导致持续增殖，但在第一次传代后分化有限，表明存在转运扩增细胞群，但具有独立的增殖和分化程序。该方法为评估小鼠气道上皮细胞的基因调控和表达提供了高保真体外模型。
Highly regulated programs for airway epithelial cell proliferation and differentiation during development and repair are often disrupted in disease. These processes have been studied in mouse models; however, it is difficult to isolate and identify epithelial cell-specific responses in vivo. To investigate these processes in vitro, we characterized a model for primary culture of mouse tracheal epithelial cells. Small numbers of cells seeded at low density (7.5 x 10(4) cells/cm(2)) rapidly proliferated and became polarized. Subsequently, supplemented media and air-liquid interface conditions resulted in development of highly differentiated epithelia composed of ciliated and nonciliated cells with gene expression characteristic of native airways. Genetically altered or injured mouse tracheal epithelial cells also reflected in vivo patterns of airway epithelial cell gene expression. Passage of cells resulted in continued proliferation but limited differentiation after the first passage, suggesting that transit-amplifying cell populations were present but with independent programs for proliferation and differentiation. This approach provides a high-fidelity in vitro model for evaluation of gene regulation and expression in mouse airway epithelial cells.