NF-KAPPA-B - A FAMILY OF INDUCIBLE AND DIFFERENTIALLY EXPRESSED ENHANCER-BINDING PROTEINS IN HUMAN T-CELLS
NF-KAPPA-B - A FAMILY OF INDUCIBLE AND DIFFERENTIALLY EXPRESSED ENHANCER-BINDING PROTEINS IN HUMAN T-CELLS
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DOI：
10.1073/pnas.87.24.10028
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发表时间：
1990-12-01
影响因子：
11.1
通讯作者：
GREENE, WC
中科院分区：
文献类型：
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作者：
MOLITOR, JA;WALKER, WH;GREENE, WC
灵敏的DNA-蛋白质交联方法已被用于表征特异性结合go κ B增强子元件的四种诱导型T细胞蛋白质（50 kDa、55 kDa、75 kDa和85 kDa）。部分蛋白水解图谱揭示了这些蛋白质的三个不同的切割模式。这些多肽在未刺激的T细胞的胞质溶胶中作为无活性前体被隔离，但可以通过佛波酯刺激在体内或通过去污剂处理在体外转化为活性形式。这些蛋白质的诱导佛波醇酯的结果在一个惊人的双相模式的核表达的55-kDa和75-kDa的物种出现在几分钟内，而50-kDa和85 kDa的物种出现后几个小时的细胞刺激。这些数据表明NF-κ B结合活性可能不对应于单一多肽，而是对应于至少四种可诱导的和差异调节的DNA结合蛋白的家族，所述DNA结合蛋白在人T淋巴细胞中以不同的动力学表达。
A sensitive DNA-protein crosslinking approach has been used to characterize four inducible T-cell proteins (50 kDa, 55 kDa, 75 kDa, and 85 kDa) that specifically bind go .kappa.B enhancer elements. Partial proteolytic mapping revealed a distinct cleavage pattern for three of these proteins. These polypeptides are sequestered as inactive precursors in the cytosol of unstimulated T cells but can be converted into active forms in vivo by phorbol ester stimulation or in vitro by detergent treatment. The induction of these proteins by phorbol ester results in a strikingly biphasic pattern of nuclear expression with the 55-kDa and 75-kDa species appearing within minutes, whereas the 50-kDa and 85 kDa species appear only several hours after cellular stimulation. These data suggest that NF-.kappa.B-binding activity may not correspond to a single polypeptide but rather a family of at least four inducible and differentially regulated DNA-binding proteins that are expressed with distinct kinetics in human T lymphocytes.