Small RNA and degradome sequencing reveals important microRNA function in Astragalus chrysochlorus response to selenium stimuli
Small RNA and degradome sequencing reveals important microRNA function in Astragalus chrysochlorus response to selenium stimuli
复制标题
DOI：
10.1111/pbi.12397
复制
发表时间：
2016-02-01
影响因子：
13.8
通讯作者：
Zhang, Baohong
中科院分区：
文献类型：
--
作者：
Cakir, Ozgur;Candar-Cakir, Bilgin;Zhang, Baohong
硒（Se）是人体必需的元素，对人类健康和环境的可持续发展起着重要作用。近年来，超富硒植物被认为是一种可供选择的硒资源。黄芪属植物是硒的超富集植物，可将硒转化为非氨基酸化合物。然而，硒代谢相关的超积累在植物中尚未阐明。microRNA（miRNAs）是通过靶向mRNA参与许多生物和代谢过程的关键分子，在植物体内硒的积累过程中也起着重要作用。在本研究中，我们鉴定了418个已知的miRNAs，属于380个家族，和151个新的miRNAs的硒暴露诱导黄绿黄芪愈伤组织。在已知的miRNAs中，287个家族在两个文库中共同表达，另外71个家族仅在硒处理的样品中表达，而60个保守家族在对照组织中表达。miR 1507 a、miR 1869和miR 2867 - 3 p在硒暴露后表达上调，miR 1507 - 5 p和miR 8781 b在硒暴露后表达下调。计算分析表明，miRNAs的靶点参与了47种细胞成分、103种分子功能和144种生物学过程。降解产物组分析显示1256个mRNA被499个miRNA靶向。因此，miR 167 a、miR 319、miR 1507 a、miR 4346、miR 7767 - 3 p、miR 7800、miR 9748和miR-n93等已知和新发现的miRNAs可能与植物激素信号转导、植物-病原菌互作和硫代谢途径有关。
Selenium (Se), an essential element, plays important roles in human health as well as environmental sustainability. Se hyperaccumulating plants are thought as an alternative selenium resource, recently. Astragalus species are known as hyperaccumulator of Se by converting it to nonaminoacid compounds. However, Se-metabolism-related hyperaccumulation is not elucidated in plants yet. MicroRNAs (miRNAs) are key molecules in many biological and metabolic processes via targeting mRNAs, which may also play an important role in Se accumulation in plants. In this study, we identified 418 known miRNAs, belonging to 380 families, and 151 novel miRNAs induced by Se exposure in Astragalus chyrsochlorus callus. Among known miRNAs, the expression of 287 families was common in both libraries, besides 71 families were expressed only in Se-treated sample, whereas 60 conserved families were expressed in control tissue. miR1507a, miR1869 and miR2867-3p were mostly up-regulated, whereas miR1507-5p and miR8781b were significantly down-regulated by Se exposure. Computational analysis shows that the targets of miRNAs are involved in different types of biological mechanisms including 47 types of cellular component, 103 types of molecular function and 144 types of biological process. Degradome analysis shows that 1256 mRNAs were targeted by 499 miRNAs. We conclude that some known and novel miRNAs such as miR167a, miR319, miR1507a, miR4346, miR7767-3p, miR7800, miR9748 and miR-n93 target transcription factors, disease resistance proteins and some specific genes like cysteine synthase and might be related to plant hormone signal transduction, plant-pathogen interaction and sulphur metabolism pathways.