Influence of different power densities of LILT on cultured human fibroblast growth
Influence of different power densities of LILT on cultured human fibroblast growth
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DOI：
10.1007/s10103-006-0379-9
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发表时间：
2006-07-01
影响因子：
2.1
通讯作者：
Marques, Marcia Martins
中科院分区：
文献类型：
--
作者：
Azevedo, Luciane Hiramatsu;de Paula Eduardo, Fernanda;Marques, Marcia Martins
本研究的目的是分析不同功率密度的低强度激光治疗（LILT）对人牙龈成纤维细胞生物调节的影响。细胞在营养缺乏的情况下培养。用GaAlAs二极管激光器（λ-660 nm，2 J/cm（2））进行激光照射。使用点式技术，以连续模式和接触方式进行两次照射，间隔12小时。实验组为：1，对照，未辐照; 11，功率为10 mW，强度为142.85 mW/cm 2; 111，29 mW和428.57 mW/cm 2。用台盼蓝拒染法测得生长曲线。照射组细胞生长明显高于对照组（P
The aim of this in vitro study was to analyze the influence of different power densities of low-intensity laser therapy (LILT) on the biomodulation of human gingival fibroblasts. The cells were cultured in nutritional deficit. Laser irradiation was carried out with a GaAlAs diode laser (lambda-660 nm, 2 J/cm(2)). The irradiation was done twice with 12-h interval using the punctual technique, at continuous mode and in contact. The experimental groups were: 1, control, nonirradiated; 11, power of 10 mW and intensity congruent to 142.85 mW/cm(2); 111, 29 mW and 428.57 mW/cm(2). Growth curves were obtained by using the trypan blue dye exclusion assay. The cell growth of the irradiated groups was significantly higher than control group (P